A rapid, simple and sensitive method for detecting mycoplasma contamination in cell cultures.

• Rapid results in 20 min
• Sensitive and specific for detecting all common mycoplasma species
• No interference with cell culture media components
• Simple operation: add reagents directly to the culture supernatant

A rapid (20 min), simple and sensitive method for detecting mycoplasma contamination in cell cultures. This innovative kit utilises the activity of mycoplasma metabolic enzymes from most mycoplasma species, including those commonly found contaminating cell cultures. Since this enzyme is not present in eukaryotic cells, the test is specific for mycoplasma. In the presence of mycoplasma, the enzymes react with the substrate to catalyse the conversion of ADP to ATP. Mycoplasma contamination can be detected by measuring the level of ATP in a sample before and after the addition of Myco-Sniff-Rapid substrate via a luciferase assay.

The kit can be used with tube luminometers, plate luminometers and multifunctional readers.