Rat Flt3 Ligand/Flt3L ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Flt3 Ligand/Flt3L in serum, plasma, tissue homogenates, and other biological fluids.

• Ready To Use ELISA kit
• Detection Range: 1,563 to 100 ng/ml
• Sensitivity: 0,938 ng/ml
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Rat Flt3 Ligand/Flt3L ELISA kit (A79825) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Flt3 Ligand/Flt3L in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Flt3 Ligand/Flt3L has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Flt3 Ligand/Flt3L present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Flt3 Ligand/Flt3L Antibody, which binds the captured Flt3 Ligand/Flt3L present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Flt3 Ligand/Flt3L captured in each well. The concentration of Flt3 Ligand/Flt3L can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.