Mouse monoclonal IgG3 antibody against human, mouse, rat SSEA-4, PE-conjugated.

• Pluripotent stem cells

The MC-813-70 antibody reacts with stage-specific embryonic antigen-4 (SSEA-4), a glycolipid carbohydrate antigen expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), undifferentiated embryonic stem (ES) and induced pluripotent stem (iPS) cells, a subset of mesenchymal stem cells, and rhesus monkey ES cell lines. No immunoreactivity is evident with undifferentiated mouse EC, EG, ES, and iPS cells. Expression of SSEA-4 is down-regulated following differentiation of human EC, ES, and iPS cells. In contrast, the differentiation of mouse EC, ES, or iPS cells may be accompanied by an increase in SSEA-4 expression. This antibody clone has been verified for labeling human ES and iPS cells grown in mTeSR™1 (Catalog #85850), and TeSR™2 (Catalog #05860), and has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit (Catalog #18166) and EasySep™ hESC/hiPSC SSEA-4 Positive Selection Kit (Catalog #18165; partial blocking may be observed).

Type:
Antigen: SSEA-4
Clonality: Monoclonal
Clone: MC-813-70
Conjugation: PE
Epitope:
Host: Mouse
Isotype: IgG3 kappa
Reactivity: Cat, Chicken, Dog, Human, Mouse, Rabbit, Rat, Rhesus monkey