Sugar free agar is a medium used for the determination of the total microbial count in food.

• Ready to use plates (90 mm) containing 22±1 ml of medium
• Light amber, very slightly opalescent
• Final pH: 7,5 ± 0,2

This medium complies with the recommendations of the international dairy federation for the enumeration of psychotrophic and mesophilic bacteria from butter and other dairy products.

Casein peptone and gelatin peptone provide amino acids, nitrogen, carbon, vitamins, and minerals for organisms growth. Sodium chloride maintains the osmotic balance of the medium. Agar is the solidifying agent. This medium does not contain any fermentable carbohydrates and has relatively poor nutrient value allowing the cultivation of the contaminant flora in dairy products.

Inoculate the medium by spread-plate or streak-plate techniques. Incubate in aerobic atmosphere for 48 hours at 35 ± 2 °C followed by further 48 hours at 20 ± 2 °C.

Disposal of waste must be carried out according to the national and local regulations in force.