The Luna® Probe one-step RT-qPCR 4X mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes.

• Simplify your reaction setup with a single-tube master mix format
• Increase sensitivity of your RT-qPCR, as 4X concentration allows for more sample input
• Maximize your throughput by multiplexing up to five targets
• Reduce risk of carryover contamination, with UDG and dUTP included in the mix
• Eliminate pipetting errors with non-interfering, visible blue tracking dye

In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.