Affinity protein expression and purification systems

Affinity protein expression and purification systems

The affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification.

pCAL vectors derived from T7 RNA polymerase-based pET vectors
Novel purification tag combines small size, gentle elution and high-affinity binding
Easy tag removal resulting in native protein

: The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately).

: For research use only. Not for use in diagnostic procedures.

Environmentally Preferable:

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Acetone ≥99%, TECHNICAL

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Sealing film for manual application, Parafilm® M

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Nitrile gloves, Kimtech™ Purple Nitrile™

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VWR® Serological Pipette, Standard Line

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Sodium hydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution

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Acetonitrile, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

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VWR® NITRILE LIGHT, Nitrile Gloves

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VWR®, Transfer Pipettes, High Performance

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Water, HiPerSolv CHROMANORM® for HPLC

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Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

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VWR®, Nitrile Gloves

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Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

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VWR® Facial Tissues

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