You searched for: Microscopy and staining reagents

Supplier: VWR Chemicals

When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.

Supplier: VWR Chemicals

VWR® Bouin tissue fixative solution.

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde in methanol 50% (v/v)).

Supplier: SIGMA ALDRICH MICROSCOPY

Glycerol alters the hygroscopic property of gelatin and its permeability to water vapour. Glycerol gelatin or glycerine jelly medium is an aqueous slide mounting media, used for mounting samples in microscopy and histology.

Supplier: VWR Chemicals

Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.

Supplier: VWR Chemicals

Safemount Q Path® is a mounting medium, supplied in an PET bottle, which is compatible with Safesolv. It glues the coverglass to the slide and fixes the probe in position after staining.

Supplier: PanReac AppliChem

Biochemical grade immersion oil for microscopy.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

Supplier: VWR Chemicals

Glue for improving adherance of histologicals sections to slides. Alternative to albumin-glycerol.

Supplier: VWR Chemicals

Cytology fixative specially designed for the conservation and transport of cytology specimens.

Supplier: VWR Chemicals

Decalcifier according to Kristenson.

Supplier: VWR Chemicals

Contains a mixture of hydrochloric acid and alcohols with some EDTA.

Supplier: Enzo Life Sciences

IHC mount is an aqueous-based permanent, high stability mounting medium designed for use with chromogenic labeling. Use for mounting of tissue specimens stained with peroxidase and alkaline phosphatase-based systems and various fluorescent dyes.

Supplier: Enzo Life Sciences

Fluoromount is an aqueous-based permanent, high stability mounting medium designed for fluorogenic labelling and ideal for long-term storage of processed tissue.

Supplier: VWR Chemicals

High viscosity mounting media for cryotomy. Less viscous than O.C.T.

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable for use with large and small mineralized (undecalcified) bone sections. Yields clear, hard blocks for cutting sections. Not water-soluble.

Supplier: Enzo Life Sciences

Antifade mounting medium (hardset) is an antifade reagent that causes little or no quenching. Use of the mounting medium significantly extends the photobleaching half-life of the fluorescent stain.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: SIGMA ALDRICH MICROSCOPY

Spurr resin is a hydrophobic resin. It is less viscous than epon mixtures and is easier to dispense. The low viscosity of the resin contributes to its penetration in tissue, mineral and dense structures. A low viscosity of 60 cps also allows easy penetration into a variety of difficult materials.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: VWR Chemicals

This medium-acting decalcifier contains a mixture of hydrochloric acids.

Supplier: VWR Chemicals

Q Path® Safesolv is a non hazardous replacement for xylene or toluene. It is compatible with the Q Path® Safemount mounting medium reference 00647520. It is used in place of xylene, toluene, methylecyclohexane or other solvents in the course of the dehydration stages of histological specimens and during the stages of staining and lamella mounting.

Supplier: VWR Chemicals

Q Path Labofix is a spray cytology fixative able to fix and store the cellular elements from gynaecological and non gynaecological smears after spreading them out on a glass slide.

Supplier: VWR Chemicals

A range of mounting media for manual and automatic mounting of slides. The Q Path® Coverquick range consists of four different mounting media with different viscosities to meet all microscopy needs. The number after the name indicates increasing viscosity: Q Path® Coverquick 1000 has the lower viscosity (around 260 mPaS) and Q Path® Coverquick 4000 has the higher viscosity (around 920 mPaS).

Supplier: Enzo Life Sciences

The IHC PAP pen provides a hydrophobic barrier when a circle is drawn around a specimen on a glass slide.

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

Supplier: VWR Chemicals

Mounting media based on isopropyl alcohol. Compatible with most solvent replacing agents. Suitable for manual and automatic mounting of slides. PET bottle of 500 ml.

Supplier: VWR Chemicals

High viscosity mounting medium for cryotomy.

Supplier: VWR Chemicals

DC1 slow acting decalcifiers contain a mixture of formic acid and formalin.