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You searched for: Western Blotting Reagents

Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Protein immunodetection reagents, SuperSignal™ West Femto maximum sensitivity substrate

Protein immunodetection reagents, SuperSignal™ West Femto maximum sensitivity substrate

Supplier: Thermo Fisher Scientific

SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.

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Horseradish peroxidase, Pierce™

Horseradish peroxidase, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

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HRP conjugates, HisProbe™

HRP conjugates, HisProbe™

Supplier: Thermo Fisher Scientific

HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.

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S-Nitrosylation western blot kit, Pierce™

S-Nitrosylation western blot kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

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Western blot enhancer, SuperSignal™, Pierce

Western blot enhancer, SuperSignal™, Pierce

Supplier: Thermo Fisher Scientific

SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.

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CN/DAB substrate kit, Pierce™

CN/DAB substrate kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.

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NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™

Supplier: Thermo Fisher Scientific

QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.

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Peroxidase conjugate stabiliser/diluent, Guardian™

Peroxidase conjugate stabiliser/diluent, Guardian™

Supplier: Thermo Fisher Scientific

Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.

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Pierce™ Western Blot Signal Enhancer

Pierce™ Western Blot Signal Enhancer

Supplier: Thermo Fisher Scientific

Western blot signal enhancer is a membrane treatment that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a Level at which it could not be detected without enhancement.

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1-Step™ Ultra TMB-Blotting solution, Pierce™

1-Step™ Ultra TMB-Blotting solution, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.

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Far-western blot kit for biotinylated proteins, Pierce™

Far-western blot kit for biotinylated proteins, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins through interaction with any biotin-labeled test protein used as the primary probe. On-membrane detection offers greater sensitivity whereas in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer.

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Protein immunodetection reagents, SuperSignal™ West Dura extended duration substrate

Protein immunodetection reagents, SuperSignal™ West Dura extended duration substrate

Supplier: Thermo Fisher Scientific

The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

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DAB substrate

DAB substrate

Supplier: Thermo Fisher Scientific

DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.

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Biotin random prime DNA labelling kit, North2South™

Biotin random prime DNA labelling kit, North2South™

Supplier: Thermo Fisher Scientific

North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.

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Amplite® West ECL HRP Substrate *Femto Sensitivity*

Amplite® West ECL HRP Substrate *Femto Sensitivity*

Supplier: AAT BIOQUEST

Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

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Chemiluminescent EMSA kit, LightShift™

Chemiluminescent EMSA kit, LightShift™

Supplier: Thermo Fisher Scientific

LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.

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1-Step™, Pierce™ Chloronaphthol

1-Step™, Pierce™ Chloronaphthol

Supplier: Thermo Fisher Scientific

Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.

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Fluorogenic peroxidase substrate kit, QuantaBlu™

Fluorogenic peroxidase substrate kit, QuantaBlu™

Supplier: Thermo Fisher Scientific

QuantaBlu™ Fluorogenic peroxidase substrate kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.

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Pierce™ Biotin 3' End DNA Labelling Kit

Pierce™ Biotin 3' End DNA Labelling Kit

Supplier: Thermo Fisher Scientific

This Biotin 3' End DNA Labelling Kit is used for tagging single-stranded DNA primers with biotin for use in non radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

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Restore™ Western Blot Stripping Buffer

Restore™ Western Blot Stripping Buffer

Supplier: Thermo Fisher Scientific

Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.

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Western Blotting Substrate, Pierce™ ECL Plus

Western Blotting Substrate, Pierce™ ECL Plus

Supplier: Thermo Fisher Scientific

The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

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SuperSignal™ Pierce™ West Pico PLUS chemiluminescent substrate

SuperSignal™ Pierce™ West Pico PLUS chemiluminescent substrate

Supplier: Thermo Fisher Scientific

SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by Western blot analysis.

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Western blotting kits, SuperSignal™ west pico chemiluminescent substrate kits, Pierce™

Western blotting kits, SuperSignal™ west pico chemiluminescent substrate kits, Pierce™

Supplier: Thermo Fisher Scientific

These kits include SuperSignal™ West Pico Substrate that is a highly sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. The kits also contain reagents for the specific detection of biotinylated antibodies, rabbit IgG or mouse IgG. The substrate’s intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.

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Western blotting blocking reagent, Amersham™ ECL™

Western blotting blocking reagent, Amersham™ ECL™

Supplier: Cytiva

Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents

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SuperSignal™ Protein ImmunoDetection Reagents, ELISA Femto Maximum Sensitivity Substrate

SuperSignal™ Protein ImmunoDetection Reagents, ELISA Femto Maximum Sensitivity Substrate

Supplier: Thermo Fisher Scientific

SuperSignal® ELISA Femto maximum sensitivity substrate utilises the SuperSignal® system with an enhancer formulation for superior protein detection and low-end linearity in ELISA applications. Ideal for automated high-throughput screening applications.

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Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit

Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit

Supplier: Thermo Fisher Scientific

The Pierce™ Chemiluminescent nucleic acid detection module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.

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DAB substrate kit, metal enhanced, Pierce™

DAB substrate kit, metal enhanced, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.

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Chemiluminescent RNA EMSA kit, LightShift™

Chemiluminescent RNA EMSA kit, LightShift™

Supplier: Thermo Fisher Scientific

LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

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Pierce™ Firefly Signal Enhancer (100X)

Pierce™ Firefly Signal Enhancer (100X)

Supplier: Thermo Fisher Scientific

Pierce™ Firefly Signal Enhancer (100X) is designed to complement the Firefly Luciferase Flash and Glow assay and is recommended when maximum sensitivity is desired.

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Pierce™ OPD (o-phenylenediamine Dihydrochloride)

Pierce™ OPD (o-phenylenediamine Dihydrochloride)

Supplier: Thermo Fisher Scientific

OPD (o-phenylenediamine dihydrochloride) is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492 nm by ELISA plate readers.

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