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You searched for: Chromatography Columns

Chromatography Columns

Chromatography columns separate molecules within mixtures during gas or liquid methods. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required. The vertical pieces are able to withstand extreme temperatures and pressure fluctuations experienced when the mobile phase is forced through the medium. Meet preparative and analytical laboratory procedures with the chromatography columns to separate dissimilar moving components based on individual polarity, composition, and size.

Solid phase extraction cartridges, Bond Elut™ SCX

Supplier: VARIAN

Bond Elut™ SCX is a strong cation exchanger with a very low pKa. Although the pKa is similar to Bond Elut™ PRS, the presence of the benzene ring in the functional group increases the potential for non-polar interactions. This non-polar characteristic becomes particularly important when conducting ion-exchange from aqueous systems, where selectivity towards compounds exhibiting cationic and non-polar character is seen.

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HPLC columns, Polaris™ amide C18

HPLC columns, Polaris™ amide C18

Supplier: VARIAN

Polaris™ amide C18 has the greatest polarity of the Polaris™ C18 family. The amide polar groups offer a different selectivity from either the C18-A or C18-ether phases. The surface has been covalently modified with alkyl amide groups and endcapped.

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SampliQ SPE cartridges

Supplier: Agilent

High quality SampliQ SPE (Solid Phase Extraction) products help the user confidently extract and concentrate samples from complex matrices, ensuring fast, accurate, and reproducible results from the very first step. The SampliQ line includes polymers, silica and non-silica options to meet user's specific SPE needs.

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Solid phase extraction cartridges, Bond Elut™ PCB

Supplier: VARIAN

Bond Elut™ PCB is a specially designed sorbent which allows for the facile extraction of polychlorinated biphenyl (PCB) compounds from a variety of matrices. Desired analytes can be loaded and eluted using a simple, single solvent method prior to analysis by GC/ECD.

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Solid phase extraction cartridges, Bond Elut™ Sodium Sulphate Drying

Supplier: VARIAN

Bond Elut™ Jr cartridges have top and bottom Luer fittings allowing easy sample processing when used in conjunction with standard SPE cartridges. Bond Elut™ LRC cartridges have a large reservoir above the sorbent bed and are suitable for use on any standard SPE vacuum manifold.

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HPLC columns, Viva biphenyl (USP L11)

HPLC columns, Viva biphenyl (USP L11)

Supplier: Restek

These biphenyl coulmns have a greater degree of dispersion than conventional phenyls and a greater degree of polarisability than phenyl hexyls, creating higher selectivity and a greater range of usability. With heightened interactions shows substantial increases in retention for dipolar, unsaturated, or conjugated solutes and enhanced orthogonal selectivity when using methanol mobile phases.

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Capillary columns, CP-Al₂O₃/Na₂SO₄

Capillary columns, CP-Al₂O₃/Na₂SO₄

Supplier: VARIAN

CP-Al₂O₃/Na₂SO₄ is an aluminum oxide PLOT column that offers high selectivity for separating ppm levels of C₁ to C₅ hydrocarbons in a main stream of C₁ to C₅ hydrocarbons. The Na₂SO₄ deactivation salt provides a polar Al₂O₃ surface, so that unsaturated compounds such as ethylene and acetylene are retained longer. These columns analyse more compounds in a single run than packed columns, while still delivering higher resolution and faster analysis times. When compared to liquid stationary phases, CP-Al₂O₃ provides increased selectivity and allows all C₁ to C₅ hydrocarbon isomers to be separated. CP-Al₂O₃ operates without the need for sub-ambient cooling.

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Solid phase extraction cartridges, Bond Elut™ ENV

Supplier: VARIAN

Bond Elut™ ENV, a PS/DVB polymer, is designed for the extraction of polar organic residues. It contains 125 μm spherical particles with a high degree of cross linking, advantageous for high volume, fast flow-through applications.

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Solid phase extraction cartridges, Bond Elut™ Alumina

Supplier: VARIAN

Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalised silica. The small particle size of the Bond Elut™ Alumina range ensures high extraction efficiency even when small bed masses are used.

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UHPLC columns, Pinnacle® DB PAH UHPLC

UHPLC columns, Pinnacle® DB PAH UHPLC

Supplier: Restek

Base-deactivated spherical silica that is optimised for UHPLC stability. Designed to resolve complex mixtures of polycyclic aromatic hydrocarbons (PAHs). Complete resolution of all 16 EPA 610 PAHs, plus two other routinely analyzed PAH compounds, in less than five minutes to greatly reduce run times and increase sample throughput.

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Solid phase extraction cartridges, Bond Elut™ CH

Supplier: VARIAN

Bond Elut™ CH is a mid-polarity sorbent that exhibits unique selectivities for certain analytes. When employed as a non-polar sorbent, CH has the approximate polarity of a C₂ sorbent. Bond Elut™ CH is often a good choice when non-polar sorbents such as C₁₈, C₈ or C₂ do not provide the desired selectivity.

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Solid phase extraction cartridges, Bond Elut™ C1

Solid phase extraction cartridges, Bond Elut™ C1

Supplier: VARIAN

Due to the methyl group and subsequent low carbon load, Bond Elut™ C1 is the least retentive of all alkyl group bonded phases for non-polar compounds. However, due to the extensive end capping of this sorbent to mask polar silanol activity, retention and elution of polar and multi-functional analytes can still be achieved. Typical applications include plasma, urine and aqueous samples.

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Solid phase extraction cartridges, Bond Elut™ PRS

Supplier: VARIAN

Bond Elut™ PRS is a strong cation exchange sorbent that is also relatively high in polarity. With no appreciable degree of hydrophobicity, in non-polar solvents, PRS is capable of polar and hydrogen bonding interactions. Due to the very low pKa of PRS, a high ionic strength eluent must be used, and so the use of PRS is recommended for weaker cationic species such as pyridinium compounds.

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Solid phase extraction cartridges, Bond Elut™ PBA

Supplier: VARIAN

PBA is a unique sorbent consisting of a phenylboronic acid covalently linked to a silica gel surface. Covalent processes result in a higher retention to the sorbent surface than other mechanisms, resulting in superior clean-up. The boronate group has a high specificity for cis-diol containing compounds such as catechols, nucleic acids, low molecular weight proteins and carbohydrates.

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Solid phase extraction cartridges, Bond Elut™ PH

Supplier: VARIAN

Bond Elut™ PH is a non-polar bonded silica material which exhibits a different selectivity to alkyl or aliphatic functionalised phases such as C8 or cyclohexyl. The electron density present in the aromatic ring affords an enhancement in the retention of conjugated or aromatic ring-containing analytes due to desirable pi-pi interactions.

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Solid phase extraction cartridges, Bond Elut™ C18

Solid phase extraction cartridges, Bond Elut™ C18

Supplier: VARIAN

Bond Elut™ C18 is the most hydrophobic, bonded silica sorbent in the Bond Elut™ range. It is the most popular SPE sorbent because of its extremely retentive nature for non-polar compounds. C18 is generally regarded as having the broadest spectrum of retention among bonded silica sorbents, since it retains most organic analytes from aqueous matrices. When analysing small to intermediate molecules, Bond Elut™ C18 can be used for desalting aqueous matrices prior to ion exchange, as salts pass through the sorbent unretained.

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Solid phase extraction cartridges, Bond Elut™ Plexa

Supplier: VARIAN

The water-wettable, hydroxylated exterior allows excellent flow, even with biological fluids. A gradient of polarity on the polymer surface shunts small analytes to the more hydrophobic centre of the polymer bead, where they are retained prior to the washing and elution steps. Plexa provides these performance enhancements due to a unique polymeric architecture with a non-retentive, hydroxylated, amide-free surface and a non-polar PS/DVB core for retaining small molecules.

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Solid phase extraction cartridges, Bond Elut™ Certify II

Supplier: VARIAN

Bond Elut™ Certify II was developed specifically for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices. Certify II is a mixed-mode cartridge packed with non-polar C8 and strong anion exchange (SAX) sorbent. It has been optimised for acidic drugs such as 11-nor-delta-9-tetrahydrocannibinol-carboxylic acid, salicylic acid, ibuprofen, acetaminophen and other compounds that possess both non-polar and anionic characteristics.

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Solid phase extraction cartridges, Bond Elut™ Certify

Supplier: VARIAN

The Bond Elut™ Certify extraction cartridge utilises a packed bed consisting of a special, non-polar C8 sorbent and a strong cation exchanger (SCX). Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for extraction of a wide range of compounds from a diverse range of aqueous matrices.

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Solid phase extraction cartridges, Bond Elut™ Diol

Supplier: VARIAN

Bond Elut™ Diol (2OH) resembles unbonded silica in its tendency for strong hydrogen bonding with analytes. 2OH can also be employed in the non-polar mode because the hydrocarbon spacer on its functional group provides enough non-polar character for retention of hydrophobic analytes. Bond Elut™ Diol is a listed SPE device for the DIN 14333-1 method on benzimidazole fungicides.

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QuEChERS dispersive kits

QuEChERS dispersive kits

Supplier: Agilent

Step 2: Dispersive SPE cleanup
Select the dispersive SPE kit suited to the type of food being analysed and the method you are following. In this step, an aliquot of the sample extract from step one (see QuEChERS extraction kits) is added to a 2 or 15 ml centrifuge tube containing a small amount of SPE sorbent and MgSO₄. The sorbent will pull out interfering matrix materials from the sample, while the MgSO₄ helps remove excess water and improve analyte partitioning.

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QuEChERS extraction kits

QuEChERS extraction kits

Supplier: Agilent

Step 1: Extraction
Choose the extraction salt packet based on your method of analysis, AOAC or EN. The buffered extraction salts are amenable for more liable pesticides. Adding solvent and then salts to a comminuted fruit or vegetable sample (10 g or 15 g) enables you to extract the pesticides of interest into the organic layer. However, adding a food sample with a high percentage of water directly to the salts may create an exothermic reaction that can affect your analyte recoveries.

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Chromatography columns with Rotaflo® stopcock, Quickfit®

Chromatography columns with Rotaflo® stopcock, Quickfit®

Supplier: SciLabware

These chromatography columns are made from PYREX®, borosilicate glass.

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Chromatography columns with Rotaflo® stopcock and ground cone, Quickfit®

Chromatography columns with Rotaflo® stopcock and ground cone, Quickfit®

Supplier: SciLabware

These chromatography columns are made from PYREX®, borosilicate glass.

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Chromatography Columns, Glass Tap/Core

Supplier: NEUBERT VOLUME GLASSWAERE

Glass, Empty columns: Yes, Ø int.: 200 mm, Length: 300 mm

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Chromatography columns with filter plates

Supplier: NEUBERT VOLUME GLASSWAERE

These chromatography columns feature a top sleeve with PTFE tap and are made from borosilicate glass 3.3.

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HPLC columns, Eurospher II, C18

Supplier: KNAUER

These columns with HPLC phase are perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks.

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Omnifit® EZ Glass Chromatography Columns for FPLC and LPLC

Supplier: OMNIFIT

EZ columns are designed to suit the majority of standard laboratory chromatography applications. They are ideal for aqueous systems and compatible with solvents used in common liquid chromatography applications such as protein purification.

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SPE cartridges

Supplier: Agilent

Bond Elut™ online SPE cartridges are designed for use with liquid chromatography (LC) to provide sample cleanup, and in certain applications, pre-concentration.

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HPLC columns, Eurospher I

Supplier: KNAUER

These endcapped Eurospher I columns are used in the determination of water soluble vitamins, steroids, catecholamines, sedatives and many other applications fields.

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