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272 results for Enzyme Assays

You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Colorimetric Xanthine Oxidase Assay Kit

Amplite® Colorimetric Xanthine Oxidase Assay Kit

Supplier: AAT BIOQUEST

Xanthine oxidase (XO)is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyse the oxidation of xanthine to uric acid.

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CPRG assay kit

CPRG assay kit

Supplier: OZ BIOSCIENCES

CPRG assay kit is designed to quantify low expression level β-Galactosidase.

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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.

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Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections.

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Amplite® Fluorimetric Pyruvate Assay Kit

Amplite® Fluorimetric Pyruvate Assay Kit

Supplier: AAT BIOQUEST

Pyruvate is an important chemical compound in intracellular metabolic pathways.

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Amplite® Fluorimetric Melanin Assay Kit

Amplite® Fluorimetric Melanin Assay Kit

Supplier: AAT BIOQUEST

Melanins have very diverse roles and functions in various organisms.

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Amplite® Colorimetric Biotin Quantitation Kit

Amplite® Colorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction (Kd = 10 to 15 M⁻¹) between a protein and its ligand.

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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.

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Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen.

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Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.

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Amplite® Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Xanthine oxidase (XO) is an enzyme that catalyses the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid.

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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

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Amplite® Colorimetric Glucose and Sucrose Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.

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Amplite® Colorimetric Oxaloacetate Assay Kit *Red Color*

Amplite® Colorimetric Oxaloacetate Assay Kit *Red Color*

Supplier: AAT BIOQUEST

Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.

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Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*

Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*

Supplier: AAT BIOQUEST

Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.

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Amplite® Colorimetric Total NAD and NADH Assay Kit

Amplite® Colorimetric Total NAD and NADH Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.

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Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Colorimetric NAD/NADH Ratio Assay Kit

Amplite® Colorimetric NAD/NADH Ratio Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Luminometric Nitroreductase Assay Kit

Amplite® Luminometric Nitroreductase Assay Kit

Supplier: AAT BIOQUEST

Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds.

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Amplite® Fluorimetric Zinc Ion Quantitation Kit

Amplite® Fluorimetric Zinc Ion Quantitation Kit

Supplier: AAT BIOQUEST

Zinc is an essential trace mineral element that plays an important role in a number of biological processes.

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Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.

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Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*

Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*

Supplier: AAT BIOQUEST

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

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Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.

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Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit

Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit

Supplier: AAT BIOQUEST

Malondialdehyde (MDA) is one of the natural byproducts during lipid peroxidation.

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Amplite® Colorimetric Endotoxin Detection Kit

Amplite® Colorimetric Endotoxin Detection Kit

Supplier: AAT BIOQUEST

Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membranes of Gram-negative bacteria.

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Amplite® Colorimetric D-Lactate Assay Kit

Amplite® Colorimetric D-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.

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Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*

Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Formaldehyde is a naturally occurring substance.

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Amplite® Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

Amplite® Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.

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