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272 results for Enzyme Assays

You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH₂ group of donors with oxygen as an acceptor.

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Amplite® Fluorimetric Xanthine Assay Kit

Amplite® Fluorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Supplier: AAT BIOQUEST

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.

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Amplite® Colorimetric Fructose-6-Phosphate Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® colorimetric fructose-6-phosphate assay kit is a highly sensitive and simple method of quantifying F6P present in a variety of samples.

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Yeast β-galactosidase assay kit, Pierce™

Yeast β-galactosidase assay kit, Pierce™

Supplier: Thermo Fisher Scientific

Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

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Amplite® Total Sulfide Quantification Kit

Amplite® Total Sulfide Quantification Kit

Supplier: AAT BIOQUEST

Hydrogen sulfide is not usually a health risk at concentrations present in household water.

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Amplite® Colorimetric Xanthine Assay Kit

Amplite® Colorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Supplier: AAT BIOQUEST

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.

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Amplite® Fluorimetric D-Lactate Assay Kit

Amplite® Fluorimetric D-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® IR

Amplite® IR

Supplier: AAT BIOQUEST

Our Amplite® IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2.

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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® fluorimetric neutrophil elastase activity assay kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity.

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Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Hydrogen peroxide (H₂O₂) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

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Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric cADP-Ribose Assay Kit

Amplite® Fluorimetric cADP-Ribose Assay Kit

Supplier: AAT BIOQUEST

This cADP-ribose assay can be performed in a convenient 96-well or 384-well microtiter plate.

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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.

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Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.

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Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyse the oxidation of monoamines.

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Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity.

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Amplite® Colorimetric  Glycerol 3-Phosphate (G3P) Assay Kit

Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit

Supplier: AAT BIOQUEST

Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway.

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Amplite® Colorimetric L-Aspartate (Aspartic Acid)  Assay Kit

Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit

Supplier: AAT BIOQUEST

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.

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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.

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Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit

Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit

Supplier: AAT BIOQUEST

Site-selective modification of proteins is of major interest in chemical biology.

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Amplite® Fluorimetric Mercuric Ion Quantitation Kit

Amplite® Fluorimetric Mercuric Ion Quantitation Kit

Supplier: AAT BIOQUEST

Mercuric ion is an essential metal ion that plays an important role in a number of biological processes.

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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections.

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Amplite® Choline Quantitation Kit

Amplite® Choline Quantitation Kit

Supplier: AAT BIOQUEST

Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis).

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Amplite® Colorimetric Ammonia Quantitation Kit *Blue Color*

Amplite® Colorimetric Ammonia Quantitation Kit *Blue Color*

Supplier: AAT BIOQUEST

Ammonia is an important source of nitrogen for living systems.

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Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.

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Quantitative peroxide assay kit (aqueous), Pierce™

Quantitative peroxide assay kit (aqueous), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

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