You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
SAM Methyltransferase assay, SAMfluoro™
Supplier: G-Biosciences
The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
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Renilla luciferase glow assay kits, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce renilla Luciferase glow assay kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of green renilla luciferase reporter.
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Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
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Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
Supplier: AAT BIOQUEST
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
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Amplite® Colorimetric Fructose-6-Phosphate Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric fructose-6-phosphate assay kit is a highly sensitive and simple method of quantifying F6P present in a variety of samples.
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Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
Supplier: AAT BIOQUEST
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
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Amplite® Colorimetric NADPH Assay Kit
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit
Supplier: AAT BIOQUEST
Malondialdehyde (MDA) is one of the natural byproducts during lipid peroxidation.
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Amplite® Fluorimetric Lipase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity.
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Amplite® Colorimetric Endotoxin Detection Kit
Supplier: AAT BIOQUEST
Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membranes of Gram-negative bacteria.
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Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Formaldehyde is a naturally occurring substance.
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Amplite® Cholesterol Quantitation Kit
Supplier: AAT BIOQUEST
Cholesterol is required to build and maintain cell membranes.
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Renilla-firefly luciferase dual assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
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Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH₂ group of donors with oxygen as an acceptor.
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Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT BIOQUEST
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Yeast β-galactosidase assay kit, Pierce™
Supplier: Thermo Fisher Scientific
Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.
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Amplite® Colorimetric Hexokinase Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Hexokinase Assay Kit is designed to accurately measure the activity of hexokinases from various sources using a simple and quick protocol.
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Quantitative peroxide assay kit (aqueous), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
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Amplite® Rapid Colorimetric Total Protein Thiol Quantitation Assay Kit
Supplier: AAT BIOQUEST
Protein thiols are very important to protein structure, protein function and biological system redox environment.
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Amplite® Colorimetric Hydrogen Peroxide Assay Kit
Supplier: AAT BIOQUEST
Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.
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Amplite® Fluorimetric DPP4 Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® fluorimetric DPP4 activity assay kit provides a simple, quick, and direct protocol for measuring DPP4 Activity.
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Amplite® Fluorimetric Tyrosinase Assay Kit
Supplier: AAT BIOQUEST
Tyrosinase is an enzyme expressed across a vast range of species from bacteria and fungi to animals.
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Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT BIOQUEST
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric Glucose and Sucrose Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® fluorimetric glucose and sucrose assay kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.
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Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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ReadiUse™ NADPH Regenerating Kit
Supplier: AAT BIOQUEST
NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).
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Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit
Supplier: AAT BIOQUEST
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
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Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Monitoring of various protease activities has become a routine task for many biological laboratories.
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Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit
Supplier: AAT BIOQUEST
Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized liver and present in blood.
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Amplite® Colorimetric Glucose Oxidase Assay Kit
Supplier: AAT BIOQUEST
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.