Stains
Overcome normal optical limitations in microscopic studies by highlighting specific structures with stains and reagents and then protect samples with fixatives. The fast-acting dyes create strong contrasts between cell components for easier detection or analyses. Sensitive attractions to certain organelle or cellular components differ among offered stains, allowing precise testing modifications. Flexible in use, ready-to-use stains may be handled manually or in conjunction with automatic equipment.
Mountant for histology, DPX, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
DPX Mountant for histology has been used as a slide mountant to fix sections.
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Sigma-Aldrich® Bouin's solution
Supplier: SIGMA ALDRICH MICROSCOPY
Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.
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Aqueous mounting medium, Fluoromount™, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.
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Aqueous mounting medium, Fluoroshield™ with DAPI, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
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Neutral mounting medium, DPX, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.
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Differentiation Solution
Supplier: SIGMA ALDRICH MICROSCOPY
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.
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Eukitt® mounting medium
Supplier: SIGMA ALDRICH MICROSCOPY
This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.
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ImmunoHistoMount™ Mounting Medium
Supplier: SIGMA ALDRICH MICROSCOPY
ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.
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Embedding medium, PolyFreeze, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.
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Aqueous mounting medium, Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
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Immersion oil for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.
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Epoxy embedding medium, hardener MNA, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator
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Hartman’s fixative histological tissue fixative, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination
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Araldite® M, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.
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Aqueous mounting medium, Fluoroshield™ with PPD, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
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Embedding kit JB-4, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
JB-4 is a water-soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. It is a technique now widely used for research and clinical diagnosis. JB-4 yields semi-thin sections (0.5μ-2μ) with excellent morphological preservation.
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