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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Inclusion bodies solubilisation buffer

Inclusion bodies solubilisation buffer

Supplier: G-Biosciences

The IBS™ buffer is specifically developed for solubilisation of inclusion bodies.

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ReadiUse™ Annexin Binding Buffer, Optimised for Imaging and Flow Cytometry

ReadiUse™ Annexin Binding Buffer, Optimised for Imaging and Flow Cytometry

Supplier: AAT BIOQUEST

The ReadiUse™ Annexin Binding Buffer *Optimised for Imaging and Flow Cytometry* is an isotonic buffer designed to promote the labeling of apoptotic cells with annexin V conjugates.

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TAE buffer, 50X

Supplier: EDVOTEK

This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.

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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer) pH 8,3

Supplier: PanReac AppliChem

Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer) pH 8,3

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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution

Supplier: PanReac AppliChem

Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.

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PBS Tablet (Phosphate Buffered Saline Tablet)

PBS Tablet (Phosphate Buffered Saline Tablet)

Supplier: AAT BIOQUEST

Each PBS tablet prepares 100 mL of a 1X PBS solution containing 137 mM sodium chloride, 10 mM phosphate buffer, and 2.7 mM potassium chloride (pH 7.4).

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pH buffer solutions, colour coded

pH buffer solutions, colour coded

Supplier: Hach

For calibration of pH meters

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Hank's balanced salt solutions (HBSS), Corning®

Hank's balanced salt solutions (HBSS), Corning®

Supplier: Corning

Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.

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Blocking solution, BLOK™ Casein

Blocking solution, BLOK™ Casein

Supplier: G-Biosciences

BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

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ReadiUse™ Yeast Cell Lysis Buffer, 5X

ReadiUse™ Yeast Cell Lysis Buffer, 5X

Supplier: AAT BIOQUEST

ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.

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TE Buffers 100X

Supplier: G-Biosciences

TE Buffer (Tris-EDTA Buffer) is a common molecular biology buffer that is used to protect DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

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Dulbecco's phosphate-buffered saline (DPBS), Corning®

Dulbecco's phosphate-buffered saline (DPBS), Corning®

Supplier: Corning

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7,0 to 7,6

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Blocking buffer, concentrate, Pierce™ Clear Milk

Blocking buffer, concentrate, Pierce™ Clear Milk

Supplier: Thermo Fisher Scientific

This clear milk blocking buffer is a long shelf life, high performance replacement for homemade milk blocking buffers in Western blotting applications.

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pH buffer, single-use, SINGLET™

pH buffer, single-use, SINGLET™

Supplier: Hach

These buffers solutions packs are used for pH meter calibration. The sealed foil pouches can be transported easily and safely without any spillage and also prevents contamination or exposure to air of the solutions.

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Buffer solution, pH 6,865

Buffer solution, pH 6,865

Supplier: PanReac AppliChem

These buffer solutions are useful for the calibration of high precision laboratory pH meters.

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Genomic DNA extraction buffers

Supplier: OMEGA BIO-TEK

EB buffer, Omega Bio-Tek

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ReadiUse™ Universal Kinase Reaction Stopping Buffer

ReadiUse™ Universal Kinase Reaction Stopping Buffer

Supplier: AAT BIOQUEST

ReadiUse™ Universal Kinase Reaction Stopping Buffer is an aqueous solution that effectively chelates divalent metal ions.

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Buffer, TE solution (TRIS-EDTA buffer) pH 8,0

Supplier: PanReac AppliChem

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

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HHBS [Hanks' Buffer with 20 mM HEPES] (100 ml)

HHBS [Hanks' Buffer with 20 mM HEPES] (100 ml)

Supplier: AAT BIOQUEST

Hank's Balanced Salt Solution with 20 mM Hepes buffer (HHBS) is used for a variety of cell culture applications, such as washing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

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Blocker™ Blocking Buffer, Casein

Blocker™ Blocking Buffer, Casein

Supplier: Thermo Fisher Scientific

Blocker™ Casein in PBS or TBS are ready-to-use, solutions of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

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ReadiUse™ Mammalian Cell Lysis Buffer *5X*

ReadiUse™ Mammalian Cell Lysis Buffer *5X*

Supplier: AAT BIOQUEST

All our ReadiUse™ reagents require minimal hands-on time.

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Buffer solution, pH 12,454

Buffer solution, pH 12,454

Supplier: PanReac AppliChem

These buffer solutions are useful for the calibration of high precision laboratory pH meters.

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Native sample loading buffer, 2X

Supplier: G-Biosciences

Loading Buffer, Native sample loading buffer (2X)

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Buffer powder pillows

Buffer powder pillows

Supplier: Hach

The unit-dose powder pillows assure buffer freshness and eliminate the possibility of contamination. Each powder pillow prepares 50 ml of buffer solutions of respective pH.

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pH buffers, DuraCal

pH buffers, DuraCal

Supplier: HAMILTON BONADUZ

For full information concerning health and safety data please contact Avantor or Hamilton directly.

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Blocking buffer, dry blend, SuperBlock™

Supplier: Thermo Fisher Scientific

SuperBlock (TBS) Blocking Buffer is an optimised TBS solution containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

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pH buffer solutions

pH buffer solutions

Supplier: PanReac AppliChem

pH Buffer solution, pH value: 7,00, Reference temperature: 20 °C

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Ready-to-use tyramide amplification buffer, 1X

Ready-to-use tyramide amplification buffer, 1X

Supplier: Biotium

For simple, optimised tyramide amplification of immunostaining in cells or tissue sections. No need to add hydrogen peroxide before use.

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Phosphate-buffered saline (PBS), Corning®

Phosphate-buffered saline (PBS), Corning®

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate

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ReadiUse™ Cell Detaching Buffer (50 ml)

ReadiUse™ Cell Detaching Buffer (50 ml)

Supplier: AAT BIOQUEST

ReadiUse™ cell detaching buffer is a cell detachment solution that does not contain mammalian or bacterial-derived products.

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