Buffers

Supplier: QIAGEN

For purification of archive-quality DNA from a wide range of sample types.

Supplier: Stemcell Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.

Supplier: Stemcell Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

Supplier: ROCHE

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

Supplier: Stemcell Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.

Supplier: Stemcell Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.

Supplier: Molekula

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

Supplier: Molekula

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Ambion

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Ambion

Cloning Buffer, Potassium acetate buffer solution pH 5,5 (3 mol/l)

Supplier: Stemcell Technologies

Dulbecco's Phosphate Buffered Saline with 2% fetal bovine serum is recommended for use as a buffer to dilute or wash nucleated cells.

Supplier: MARKET SOURCE PART PROCESS

Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)

Supplier: Stemcell Technologies

Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.

Supplier: Molekula

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Ambion

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

Supplier: Stemcell Technologies

Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.