Assays

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Supplier: VWR Chemicals

Fluorescent DNA Quantitation Kit uses fluorescent bisBenzimide H33258 dye to bind to AT sequences of the minor groove of double-stranded DNA. In the assay, the sample is excited at 360 nm and when the dye binds to dsDNA an emission spectrum at 460 nm is produced.

Supplier: VWR Chemicals

The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.

Supplier: VWR Chemicals

The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

Supplier: Abcam

Rat alpha 2 Macroglobulin ELISA kit ab157730 is used to quantify Rat alpha 2 Macroglobulin with a sensitivity of 1.795 ng/ml. Sandwich ELISA kit.

Supplier: Abcam

Mouse Prolactin/PRL ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Prolactin/PRL with a sensitivity of 139 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader.

Supplier: Biosensis

Biosensis proBDNF (Brain-derived neurotrophic factor, BDNF, Abrineurin) RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples.

Supplier: Abcam

Human KAT3B / p300 ELISA kit is a single-wash 90-min Simplestep used to quantify Human KAT3B / p300 with a sensitivity of 51 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair.

Supplier: Abcam

Human Alpha-synuclein (phospho S129) Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Alpha-synuclein (phospho S129).

Supplier: Abcam

Human Protein S Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Protein S.

Supplier: Enzo Life Sciences

The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

Supplier: Enzo Life Sciences

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

Supplier: Abcam

Mouse Amphiregulin ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Amphiregulin in Mouse in plasma, cell culture supernatant, Serum samples.

Supplier: Abcam

Mouse GDF-15 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GDF-15 with a sensitivity of 22,2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader.

Supplier: Abcam

Human BCA1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human BCA1 in Human in Plasma, Cell culture supernatant, Serum samples.