Next Generation Sequencing Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

Rapid Adapter Auxiliary V14

Supplier: Oxford Nanopore Technologies

Additional rapid adapter and adapter buffer to enable customers to maximise the use of rapid barcoding kits 24 and 96 V14 (SQK-RBK114.24 and SQK-RBK114.96).

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DNA Library Prep Kit, sparQ

Supplier: Quantabio

An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

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Flow Cell Priming Kit

Supplier: Oxford Nanopore Technologies

Additional flow cell priming reagents to enable additional loading of more library during a sequencing run.

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Ligation Sequencing Kits

Supplier: Oxford Nanopore Technologies

Ligation Sequencing Kits enable ligation-based, PCR-free nanopore sequencing of native DNA.

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DNA fragment and library prep kit, sparQ

Supplier: Quantabio

The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.

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Next generation sequencing (NGS), DNA/RNA clean up Mag-Bind® TotalPure

Supplier: OMEGA BIO-TEK

Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent to sample ratio used, giving the user flexibility to perform left, right or double-sided size selection.

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SFB expansion

Supplier: Oxford Nanopore Technologies

Short Fragment Buffer (SFB).

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Long Fragment Buffer Expansion

Supplier: Oxford Nanopore Technologies

Additional Long Fragment Buffer (LFB).

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Rapid PCR Barcoding Kit 24 V14

Supplier: Oxford Nanopore Technologies

The Rapid PCR Barcoding Kit 24 V14 provides a fast and simple method of preparing up to 24 barcoded samples for sequencing 1-5 ng of gDNA using PCR.

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PCR Master Mix, sparQ HiFi

Supplier: Quantabio

sparQ HiFi PCR Master Mix was specially designed for high efficiency library amplification from low DNA input. sparQ HiFi PCR Master Mix minimises the number of amplification cycles required to achieve the threshold required for sequencing. The result is a superior performing high fidelity master mix that increases library yield which reduces PCR-derived artifacts for a variety of sequencing applications.

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PCR expansion

Supplier: Oxford Nanopore Technologies

PCR Primers (PRM) and PCR Adapters (PCA).

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Post-Bisulfite DNA Library Preparation Kit (For Illumina®), Abcam

Supplier: Abcam

ab185906 is a complete set of optimized reagents to prepare a DNA library - after successful bisulfite conversion - for various Illumina® platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representative bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications.

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Microbial Amplicon Barcoding Kit 24 V14

Supplier: Oxford Nanopore Technologies

Amplification and full-length sequencing of 16S rRNA for bacterial profiling and ITS for fungal profiling, with barcoding for up to 24 samples.

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Control Expansion

Supplier: Oxford Nanopore Technologies

DNA or RNA for sequencing control experiments.

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Midnight RT PCR Expansion

Supplier: Oxford Nanopore Technologies

An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.