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You searched for: Cell Culture Supplements and Additives

Cell Culture Supplements and Additives

The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.

Corning® Matrigel® Basement Membrane Matrix, Corning

Corning® Matrigel® Basement Membrane Matrix, Corning

Supplier: Corning

Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.

    
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Corning® Nu-Serum™ Serum Replacements, Corning

Corning® Nu-Serum™ Serum Replacements, Corning

Supplier: Corning

Corning® Nu-Serum™ growth media supplement provides a low-protein alternative to newborn calf, foetal bovine, and other sera routinely used for cell culture. The low-protein content facilitates protein purification, virus production, monoclonal antibody production and screening, and increases the frequency of successful transfection of cells.

    
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Corning® Cell-Tak™ Cell and tissue adhesive

Corning® Cell-Tak™ Cell and tissue adhesive

Supplier: Corning

Corning® Cell-Tak™ Cell and Tissue Adhesive is a formulation of polyphenolic proteins extracted from Mytilus edulis (marine mussel). These proteins are the key components of the glue secreted by the mussel to anchor itself to solid substrates in the marine environment. Corning® Cell-Tak™ is used to attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, PTFE, and biological materials. It can simplify the manipulation of biological samples for a number of in vitro techniques, including in situ hybridisation, immunoassays, microinjection, immunohistochemistry, and establishing primary cells in culture. Corning® Cell-Tak™ is biocompatible and demonstrates no species specificity.

    
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Multiwell plates, Corning® Matrigel® basement membrane matrix

Multiwell plates, Corning® Matrigel® basement membrane matrix

Supplier: Corning

6-well plates, coated, PS, non-sterile, flat bottom, Corning® Matrigel®

    
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HAT (Hypoxanthine, Aminopterin, Thymidine), Corning®

HAT (Hypoxanthine, Aminopterin, Thymidine), Corning®

Supplier: Corning

HAT Medium (hypoxanthine-aminopterin-thymidine medium) is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells

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SC substrate, Corning® Synthemax® II

SC substrate, Corning® Synthemax® II

Supplier: Corning

Synthemax® II-SC Substrate is an easy to use, self-coating material for creating a unique synthetic surface that mimics the natural cell environment. This surface supports the adhesion and expansion of stem cells in their undifferentiated state and directs differentiation into specialised cell types. In addition, it offers a synthetic, xeno-free alternative to biological coatings and/or feeder cell layers used in hES and iPS cell culture, whenever needed and appropriate.

    
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Middlebrook 7H10 OADC (Oleic Albumin Dextrose Catalase) Growth Supplement

Supplier: US Biological

Enrichment supplement recommended for the cultivation of Mycobacteria sp. OADC enrichment medium is used with Middlebrook 7H10 Mycobacteria Agar (Powder).

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Trace element solution 2, MP Biomedicals

Supplier: MP Biomedicals

Trace Element Solution 2 is a 1000X sterile filtered solution, suitable for use with serum-free media; ideal for plant cell culture.

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Protease inhibitor cocktail, MP Biomedicals

Supplier: MP Biomedicals

A broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors such as AEBSF, Sodium EDTA, Leupeptin and Pepstain A.

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Protease inhibitor mix

Protease inhibitor mix

Supplier: Cytiva

Sample preparation often requires the inhibition of protease activity. Protease inhibitor mix combines competitive and non competitive protease inhibitors, which protect proteins from proteolysis during purification from animal tissues, plant tissues, yeast and bacteria.

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Cell culture supplement, Basal Medium Eagle (BME) Vitamin Concentrate (100X), MP Biomedicals

Supplier: MP Biomedicals

Basal Medium Eagle (BME) Vitamin Concentrate (100X), without L-glutamine

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Cell detachment solution, Accutase, MP Biomedicals

Supplier: MP Biomedicals

Accutase solution is a trypsin replacement that contains both proteolytic and collagenolytic enzymes for cell detachment.

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Polyribonucleotide poly(I)·poly(C)

Polyribonucleotide poly(I)·poly(C)

Supplier: Cytiva

The double-stranded polyribonucleotide poly(I)·poly(C) induces interferon in rabbit cells, and there has been interest in its effectiveness for inducing human interferon in vitro.

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Lymphocyte Separation Medium

Lymphocyte Separation Medium

Supplier: Tonbo Biosciences

Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood.

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Epidermal growth factor (EGF) from E.coli, MP Biomedicals

Supplier: MP Biomedicals

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells.

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Brewers yeast, MP Biomedicals

Brewers yeast, MP Biomedicals

Supplier: MP Biomedicals

Brewers yeast is often used as a source of B-complex vitamins, chromium, and selenium. It is also can be used to bake bread, ferment wine, beer and ethanol production.

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BIOFLOAT™ FLEX Coating Solution

BIOFLOAT™ FLEX Coating Solution

Supplier: FACELLITATE

The BIOFLOAT™ FLEX coating solution guarantees the creation of surfaces without unspecific protein or cell binding.

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MEM Eagle non-essential amino acids (NEAA) concentrate (100X solution), MP Biomedicals

Supplier: MP Biomedicals

Most cells can synthesise non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration levels are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.

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FastGro synthetic, animal free, chemically defined FBS replacement, sterile, MP Biomedicals

FastGro synthetic, animal free, chemically defined FBS replacement, sterile, MP Biomedicals

Supplier: MP Biomedicals

FastGro allows culturing cells in vitro without the use of serum and without any animal or human derived compound. FastGro is a fully defined additive, avoiding the need for serum, for a wide range of cell culture practices. Since FastGro is chemically defined it avoids lot-to-lot variations. All components in FastGro are highly purified and identified chemical compounds.

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Egg yolk tellurite emulsion for microbiology, Millipore®

Supplier: Merck

Sterile stabilized tellurite emulsion of egg yolk recommended for the identification of Staphylococcus species.

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Cell detachment solution, Accumax™

Supplier: Merck

Accumax™ is a cell detachment solution consisting of proteolytic and collagenolytic enzymes.

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Rat tail collagen coating solution

Supplier: Merck

Rat tail collagen coating solution enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. Commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types. This collagen solution is prepared in 0,02 M acetic acid at 50 ug/ml and sterile filtered.

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Sigma-Aldrich® Select yeast extract, BioReagent, suitable for cell culture

Supplier: Merck

Select Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.

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CellAdhere™ Laminin-521

CellAdhere™ Laminin-521

Supplier: STEMCELL Technologies

Matrix for maintenance of human ES and iPS cells in combination with TeSR™ maintenance media.

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DisCoZyme 1, ACF

DisCoZyme 1, ACF

Supplier: STEMCELL Technologies

Collagenase/dispase blend with high collagenase and high caseinase activity.

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CellAdhere™ Type I Collagen, Bovine, Solution

CellAdhere™ Type I Collagen, Bovine, Solution

Supplier: STEMCELL Technologies

Support cell attachment by preparing collagen-coated tissue cultureware or 3D collagen gels using CellAdhere™ Type I Collagen, Bovine, Solution. The most abundant type of collagen in the human body, type I collagen is a natural extracellular matrix protein that provides structural support and mimics physiological conditions, making it an ideal cultureware coating surface to support cell attachment.

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Collagenase Type I (0,25%)

Collagenase Type I (0,25%)

Supplier: STEMCELL Technologies

Cell dissociation reagent.

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Elastase

Elastase

Supplier: STEMCELL Technologies

For digestion of native elastin.

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Collagenase Type V

Collagenase Type V

Supplier: STEMCELL Technologies

For digestion of native collagen fibrils.

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Animal Component-Free Cell Dissociation Kit

Animal Component-Free Cell Dissociation Kit

Supplier: STEMCELL Technologies

Dissociation kit for human stem and progenitor cells.

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