424 Results for: "chromatography preparative"

Supplier: Cytiva

ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.

Supplier: MP Biomedicals

This product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins.Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region.

Supplier: Resonac

SB-800 HQ are polyhydroxymethacrylate-based SEC columns for the molecular weight distribution analysis of water-soluble polymers. The solvent can be substituted with DMF (except for SB-802 HQ and SB-807 HQ columns), enabling the analysis of polar polymers.

Supplier: VWR Collection

Experience the next level of high-performance liquid chromatography with the Chromaster Plus HPLC system. This latest version boasts a sleek new design and enhanced performance features, including the upgraded CM5310 column oven and CM5410 UV detector. The new design offers a modern and user-friendly interface, while the enhanced performance of the CM5310 column oven and CM5410 UV detector ensures superior results. Additionally, users can enjoy peace of mind with an extended warranty coverage.

Supplier: MP Biomedicals

Product is rabbit IgG (whole molecule) covalently coupled to Sepharose-4B, and packed in a polystyrene chromatography column.IgG affinity gel contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because this gel is not a bind-and-release support, it is extremely fast and gentle to antibodies, resulting in antibody preparations of high purity and high activity. IgG affinity gel works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with this gel are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.

Supplier: MP Biomedicals

Sodium phosphate dibasic heptahydrate is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography. Sodium phosphate dibasic is highly hygroscopic and water soluble. Useful in conjunction with Sodium Phosphate, Monobasic in the preparation of biological buffers.

Supplier: Cytiva

For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.

Supplier: GRANT INSTRUMENTS

A range of eco-friendly, refrigerated/heating circulating baths with an intuitive user-interface and sleek design. Units offer significant running cost savings whilst delivering powerful cooling when needed. Choice of 2 models with temperature range of –25 to +150 °C (model dependent*). Ideal for applications in pharmaceutical, education, industrial and life science areas for use with mini pilot plant reactors, cooling rotary evaporators, replacing running tap water cooling, immersing small samples, photometry, chromatography systems, sample preparation, general cooling, reaction chemistry, temperature control, semi-conductor manufacturing, rheometry, refractometry and electrophoresis cooling.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Supplier: Cytiva

CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Supplier: MERKLINGER PRAZISION WERKZEUG

This column-packing technology is based on a combination of vacuum suction with subsequent column bed compression that stabilises the packing. A common water-jet pump is used to generate the vacuum. The sorbent slurry is sucked through a packing reservoir into the column by applying low pressure at the bottom end of the column.

Supplier: DR MAISCH

Semi-preparative and preparative spherical silica material allows the clean-up of raw products from mg up to kg amounts, depending on the column ID and length. Spherical materials are easy to pack in columns.

Supplier: Cytiva

HiTrap™ Protein G HP affinity columns are pre-packed with 1 or 5 ml of Protein G Sepharose™ high performance for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.

Supplier: Cytiva

Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.

Supplier: Aviva Systems Biology

This product was prepared from normal serum by delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipi 1 * 1 mg

Supplier: G-Biosciences

ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting 1 * 50 mL

Supplier: G-Biosciences

ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting 1 * 15 mL

Supplier: Restek

Sample Preparation in Chromatography Author: SC Moldoveanu and V David ISBN 0- 1 * 1 items

Supplier: Restek

Scale-Up Optimization in Preparative Chromatography Authors:AS Rathore and A V 1 * 1 items

Supplier: DWK Life Sciences

KONTES FLEX-COLUMN Chromatography Columns, Kimble Chase, Preparative, 74 mL, 25 x 150 mm 1 * 5 items

Supplier: G-Biosciences

sodium phosphate monobasic solution [nah2po4] is used to prepare phosphate based biological buffers which are used in molecular biol ogy, biochemistry and chromatography 1 * 100 mL

Supplier: G-Biosciences

sodium phosphate monobasic solution [nah2po4] is used to prepare phosphate based biological buffers which are used in molecular biol ogy, biochemistry and chromatography 1 * 500 mL

Supplier: G-Biosciences

sodium phosphate dibasic solution [na2hpo4] is used to prepare phosphate based biological buffers which are used in molecular biolog y, biochemistry and chromatography 1 * 500 mL

Supplier: GILSON LCMS

Rack, code 22, holds quantity of 44, 18×150 mm tubes (25 ml), for use with FC204

Supplier: G-Biosciences

sodium phosphate dibasic solution [na2hpo4] is used to prepare phosphate based biological buffers which are used in molecular biolog y, biochemistry and chromatography 1 * 100 mL

Supplier: EDVOTEK

affinity chromatography of glucose binding proteins 1 * 1 items

Supplier: BUCHI

Flash chromatography system

Supplier: BUCHI

PURE chromatography systems for flash applications.

Supplier: VWR Chemicals

Dichloromethane stabilised, HiPerSolv CHROMANORM® for preparative HPLC

Supplier: VWR Chemicals

For preparative HPLC