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You searched for: buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Cell lysis analysis kit, SideStep II

Supplier: AGILENT

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer

Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer

Supplier: Thermo Fisher Scientific

Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

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Blocker™ Blocking Buffer, Casein

Blocker™ Blocking Buffer, Casein

Supplier: Thermo Fisher Scientific

Blocker™ Casein in PBS or TBS are ready-to-use, solutions of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

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CytoWatch™ Multiplexing Buffer for Flow Cytometry, 20X

CytoWatch™ Multiplexing Buffer for Flow Cytometry, 20X

Supplier: AAT Bioquest

CytoWatch™ Multiplexing Buffer is a buffer optimised for the multicolor flow cytometric analysis of cells.

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ReadiUse™ Cell Detaching Buffer (50 ml)

ReadiUse™ Cell Detaching Buffer (50 ml)

Supplier: AAT Bioquest

ReadiUse™ cell detaching buffer is a cell detachment solution that does not contain mammalian or bacterial-derived products.

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Lysis buffer, CTAB

Lysis buffer, CTAB

Supplier: PanReac AppliChem

Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

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Pierce TMT™ Elution Buffer

Pierce TMT™ Elution Buffer

Supplier: Thermo Fisher Scientific

Thermo Scientific TMT Elution Buffer is for use with Thermo Scientific Immobilized Anti-TMT Resin to enrich samples for peptides that have been chemically labeled with Tandem Mass Tag™ (TMT™) Reagents.

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Blocking buffers, protein-free, Pierce™

Blocking buffers, protein-free, Pierce™

Supplier: Thermo Fisher Scientific

Pierce Protein-free blocking buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

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RIPA lysis and extraction buffer, Pierce™

RIPA lysis and extraction buffer, Pierce™

Supplier: Thermo Fisher Scientific

The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

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Blocking buffer, Blocker™ BSA (10X) in PBS or TBS

Blocking buffer, Blocker™ BSA (10X) in PBS or TBS

Supplier: Thermo Fisher Scientific

Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

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Buffers, sodium phosphate buffer solutions (0,5 mol/L)

Supplier: Cayman Chemical

Sodium phosphate stock solution (0,5 mol/L), pH value: 7,0

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ReadiUse™ Annexin Binding Buffer, Optimised for Imaging and Flow Cytometry

ReadiUse™ Annexin Binding Buffer, Optimised for Imaging and Flow Cytometry

Supplier: AAT Bioquest

The ReadiUse™ Annexin Binding Buffer *Optimised for Imaging and Flow Cytometry* is an isotonic buffer designed to promote the labeling of apoptotic cells with annexin V conjugates.

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Extraction buffers, FOCUS™

Extraction buffers, FOCUS™

Supplier: G-Biosciences

FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

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Luciferase cell lysis buffer (2X), Pierce™

Luciferase cell lysis buffer (2X), Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.

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Phosphate-buffered saline (PBS), Corning®

Phosphate-buffered saline (PBS), Corning®

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate

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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution

Supplier: PanReac AppliChem

Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.

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VWR®, Eluent buffers for amino acid analyzer

VWR®, Eluent buffers for amino acid analyzer

Supplier: VWR Chemicals

Regenerating F, Li

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SSC buffer (20X)

Supplier: Thermo Fisher Scientific

Hybridisation Buffer, SSC buffer, 20X

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ReadiUse™ Yeast Cell Lysis Buffer, 5X

ReadiUse™ Yeast Cell Lysis Buffer, 5X

Supplier: AAT Bioquest

ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.

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Dulbecco's phosphate-buffered saline (DPBS), Corning®

Dulbecco's phosphate-buffered saline (DPBS), Corning®

Supplier: Corning

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7,0 to 7,6

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ReadiCleave™ AML Cleavage Buffer, 5X Aqueous Solution, AAT Bioquest

Supplier: AAT Bioquest

ReadiCleave™ AML Cleavage Buffer provides an optimised formula to effectively remove the fluorescence tags from ReadiCleave™ AML-labeled biological targets when needed.

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Ready-to-use tyramide amplification buffer, 1X

Ready-to-use tyramide amplification buffer, 1X

Supplier: Biotium

For simple, optimised tyramide amplification of immunostaining in cells or tissue sections. No need to add hydrogen peroxide before use.

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J.T.Baker® DILUT-IT™, Buffer Solutions, Concentrate

Supplier: Avantor

Volumetric concentrate, for dilution to 500 ml.

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Tris-Borate-EDTA (TBE) buffer, 10X, Molecular biology grade

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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Binding and elution buffers for antibody purification, Pierce™

Binding and elution buffers for antibody purification, Pierce™

Supplier: Thermo Fisher Scientific

These buffers ensure high-yield, non denaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

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ReadiUse™ Viral RNA Lysis Buffer

ReadiUse™ Viral RNA Lysis Buffer

Supplier: AAT Bioquest

Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.

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Phosphate-Buffered saline (PBS), 10X concentrate, pH 7,4, molecular biology grade, Corning®

Phosphate-Buffered saline (PBS), 10X concentrate, pH 7,4, molecular biology grade, Corning®

Supplier: Corning

Phosphate Buffered Saline (PBS) is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research

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TES buffer, 1X, ready to use, pH 7,8

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1,0 mM EDTA at a pH of 7,8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionised water.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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MOPS buffer, powder, Corning®

MOPS buffer, powder, Corning®

Supplier: Corning

Running Buffer, MOPS buffer, powder, 100 g

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