buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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J.T.Baker® DILUT-IT™, Buffer Solutions, Concentrate

Supplier: Avantor

Volumetric concentrate, for dilution to 500 ml.

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J.T.Baker® BAKER ANALYZED®, Buffer Solutions

Supplier: Avantor

Ready-to-use pH buffers are manufactured in large lots to stringent specifications and utilise quality control procedures to reduce lot-to-lot variability. You can eliminate the time and expense of preparation and standardisation of solutions, helping streamline high-volume analytical work in applications such as quality control.

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VWR®, pH electrode maintenance kit

Supplier: VWR Chemicals

The measurement of the pH value is one of the most frequently and universally made measurements in science.

What is often not fully understood is the fact that a large number of problems related to pH measurement is the
correct selection, care or maintenance of the electrodes with particular emphasis on the reference electrode.

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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VWR® UltraPure, Phosphate buffered saline (PBS), powdered

Supplier: VWR Chemicals

PBS, Ultra Pure Grade is a powdered, magnesium- and calcium-free formulation of PBS suitable for cell culture and for use in flow cytometry applications.

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VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR®, Hybridisation buffers with 50% formamide

Supplier: VWR Chemicals

Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.