141 Results for: "TriLink BioTechnologies"
TriLink® CleanCap® FLuc mRNA
Supplier: TriLink BioTechnologies
The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
Expand 3 Items
TriLink® CleanCap® Reagent AG (3'OMe)
Supplier: TriLink BioTechnologies
TriLink's patented CleanCap® Reagent AG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5' capped mRNA resulting in a Cap 1 structure with >95% capping efficiency.
Expand 4 Items
TriLink® CleanCap® Reagent AG
Supplier: TriLink BioTechnologies
CleanCap® Reagent AG is TriLink’s patented co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. CleanCap has shown to provide up to 98% capping efficiency.
Expand 5 Items
TriLink® N1-Methyl-Pseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
N1-Methyl-Pseudouridine-5'-Triphosphate is widely used in mRNA synthesis for therapeutic and vaccine development to improve mRNA functionality. This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.
Expand 4 Items
N,N-Dimethylformamide, anhydrous
Supplier: TriLink BioTechnologies
N,N-Dimethylformamide, anhydrous
Expand 1 Items
TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)
Expand 3 Items
TriLink® 2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.
Expand 3 Items
TriLink® N1-Propylpseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® N1-Propylpseudouridine-5'-Triphosphate
Expand 4 Items
TriLink® CleanCap® Renilla Luc mRNA (5 moU)
Supplier: TriLink BioTechnologies
Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.
Expand 3 Items
Digoxigenin kits, ChromaLink™ Digoxigenin One-Shot antibody labelling kit
Supplier: TriLink BioTechnologies
ChromaLink™ Didoxigenin contains a UV-traceable chromophore to enable reproducibility labelling. The degree of biotinylation can be measured in minutes, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan, biotin incorporation can be quantified and reproducible production of consistent batches ensured. ChromaLink™ labelling can be paired with NanoLink™ Streptavidin Magnetic Beads for many types of assay development.
Expand 2 Items
TriLink® 2'-Deoxy-P-nucleoside-5'-Triphosphate
Supplier: TriLink BioTechnologies
2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.
Expand 3 Items
TriLink® 5-Propargylamino-2'-deoxycytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 5-Propargylamino-2'-deoxycytidine-5'-Triphosphate
Expand 3 Items
TriLink® 2'-O-Methylcytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 2'-O-Methylcytidine-5'-Triphosphate
Expand 3 Items
Accessories for Solulink™ kits
Supplier: TriLink BioTechnologies
Protein assay, Zeba desalting columns
Expand 6 Items
TriLink® 2'-O-Methyluridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 2'-O-Methyluridine-5'-Triphosphate
Expand 3 Items
CleanCap® AG (3′ OMe) CleanScript™ IVT Kit
Supplier: TriLink BioTechnologies
TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 of 100 µl reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes including CleanScribe RNA polymerase, 10X CleanScript IVT reaction buffer, and a linearized FLuc DNA control template.
Expand 1 Items
Biotinylation kits, ChromaLink™ One-Shot antibody labelling kit
Supplier: TriLink BioTechnologies
ChromaLink™ Biotin contains a UV-traceable chromophore to enable reproducibility labelling. The degree of biotinylation can be measured in minutes, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan, biotin incorporation can be quantified and reproducible production of consistent batches ensured. ChromaLink™ labelling can be paired with NanoLink™ Streptavidin Magnetic Beads for many types of assay development.
Expand 1 Items
TriLink® Uridine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
TriLink® Uridine-5'-O-(1-Thiotriphosphate)
Expand 3 Items
TriLink® 2'-Deoxycytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.
Expand 2 Items
TriLink® 2'-Deoxyguanosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.
Expand 2 Items
TriLink® Biotin-16-Aminoallyluridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA.
Expand 3 Items
TriLink® Biotin-7-Deaza-7-Propargyl-dGTP
Supplier: TriLink BioTechnologies
TriLink® Biotin-7-Deaza-7-Propargyl-dGTP
Expand 3 Items
Antibody labelling kits, Fluorescein one-shot antibody labelling kit
Supplier: TriLink BioTechnologies
Fluorescein Antibody Conjugation kit offers an efficient method that delivers >95% conversion of antibody to conjugate. The kit includes everything needed to make two direct-labelled fluorescein-antibody conjugates from 100 µg of any user-supplied antibody.
Expand 1 Items
TriLink® N1-Methyl-2'-O-Methylpseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® N1-Methyl-2'-O-Methylpseudouridine-5'-Triphosphate
Expand 3 Items
TriLink® Cytidine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
TriLink® Cytidine-5'-O-(1-Thiotriphosphate)
Expand 3 Items
TriLink® Biotin-16-Aminoallyl-2'-deoxyuridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
Biotin-16-Aminoallyl-2'-deoxyuridine can be enzymatically incorporated into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this NTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.
Expand 2 Items
Antibody labelling kits, Antibody-Oligonucleotide All-in-One conjugation kit
Supplier: TriLink BioTechnologies
The Antibody-Oligonucleotide All-in-One Conjugation kit offers TurboLink™ catalysed conjugation based on Solulink’s proprietary linking technology to prepare antibody-oligonucleotide conjugates without chromatography. The kit includes buffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).
Expand 1 Items
CleanCap® AU FLuc saRNA
Supplier: TriLink BioTechnologies
This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the firefly Photinus pyralis, as self-amplifying RNA (saRNA). FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
Expand 6 Items
Biotinylation kits, ChromaLink™ Biotin Antibody Labelling kit
Supplier: TriLink BioTechnologies
ChromaLink™ Biotin Protein Labelling kit offers a complete solution for the quantitative biotinylation of proteins from 20 kDa to 200 kDa and from 25 µg to 1 mg. The kit incorporates traceable biotin on proteins and allows for direct spectrophotometric verification of biotin incorporation. A simple Å280 and Å354 measurement determines the number of biotins per protein molecule.
Expand 1 Items
Conjugation kits, protein-protein conjugation kit
Supplier: TriLink BioTechnologies
The Protein-Protein Conjugation kit is designed to easily and efficiently conjugate two proteins together. This kit is flexible so that researchers with little or no conjugation experience can make their own custom protein-protein conjugates to suit their needs. It includes all of the necessary components - including S-HyNic and S-4FB - and protocols for easy and specific crosslinking of any protein with any other protein of a different size greater than 7 kDa.