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Acid Phosphatase, Leukocyte (TRAP) Kit Kit formulated with all liquid reagents, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Kit is used to demonstrate acid phosphatase and tartrate resistant acid phosphatase (TRAP) in blood, bone marrow and tissue touch preparations.

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Naphthol AS-BI phosphate solution 12,5 mg/mL, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Naphthol AS-BI Phosphoric acid solution for use in the Sigma Acid Phosphatase (TRAP) Procedure, Kit 387A.

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Methyl green zinc chloride salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl green zinc chloride salt, Sigma-Aldrich®

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Carmine, powder, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Carmine is a red compound and is generally used with aluminum, iron and other metal salts to enhance its activity. During smear preparation, it is required for chromatin staining in fresh material. In presence of aluminum chloride, it is used for the staining of glycogen.

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Sudan II 90% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Sudan II has been used to determine its presence in commercial spices such as hot chilli food samples (LC–ESI–MS/MS).

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Haematoxylin solution Gill III, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time. Used with hematoxylin and eosin staining.

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Giemsa's stain modified, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

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Naphthol AS-GR phosphate disodium salt

Supplier: SIGMA ALDRICH MICROSCOPY

Produces highly insoluble dark green stain in histochemical studies.

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1-Methoxy-5-methylphenazinium methyl sulphate ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) is a photochemically stable electron-transport mediator between NAD(P)H and tetrazolium dyes. The reduced form of MPMS converts tetrazolium salt to purple precipitate formazan. It is a very stable and non-enzymic electron carrier that mediates electron transfer by dehydrogenases in vitro to various electron acceptors such as tetrazolium dyes or the electrode of an enzymic electric cell.

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Fluorescent Brightener 28, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps.

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Acid blue 80 40% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Blue 80, Dye content 40 %

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Aqueous mounting medium, Fluoroshield™, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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3,3'-Diaminobenzidine (DAB) 97% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3,3′-Diaminobenzidine has been used as a chromogen for the visualisation in immunohistochemistry of chondrosarcoma sections, mammary carcinomas and brain tissue sections. Peroxidase substrate; reagent for spectrophotometric determination of selenium. DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualisation of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidised DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures.

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Acetone 2,4-Dinitrophenylhydrazone 99% analytical standard, for environmental analysis, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acetone 2,4-Dinitrophenylhydrazone 99% analytical standard, for environmental analysis, Sigma-Aldrich®

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N-(5-Aminopentyl)biotinamide trifluoroacetate salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Biotinylation reagent

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Tartrazine, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Yellow counterstain suitable for a wide range of histological staining procedures.

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Formalin 10% histological fixative, neutral buffered, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Formalin solution (10%, neutral buffered) is a widely used fixative for samples processed for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

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Tetrazolium violet, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tetrazolium Violet is a monotetrazolium salt which forms water-insoluble formazans. These salts are mainly used as indicators of extracellular redox activity and cell redox potential. Tetrazolium violet is positively charged and cell-permeable.

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Ren™ HY 956 epoxy embedding medium, hardener, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

to be used with SIAL 78711 RenLam M-1 (Epoxy Embedding Medium)

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Crystal violet solution Grams for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.

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Acid Fuchsin calcium salt ≥60 % (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Fuchsin calcium salt,certified by the Biological Stain Commission, Dye content ≥60 % (Acid Magenta; Acid Rubin; Fuchsin S, SS, SS, ST, or SIII; Rubin S)

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2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

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4,4'-Bis(dimethylamino)diphenylmethane 98%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4,4′-Methylenebis(N,N-dimethylaniline) is an intermediate in dye manufacture and works as a reagent for the determination of lead. It is suitable for the qualitative assay of cyanogens.

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Alginic acid (from Macrocystis pyrifera (kelp)), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Alginic acid is a high molecular weight linear polymer isolated from seaweed. It is a linear β-1→4-linked polymer of n-mannuronic and L-guluronic acid. It is an acid polysaccharide present in the extracellular matrix of brown algae.

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Calcein blue fluorescent dye, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Calcein blue fluorescent dye, Sigma-Aldrich®

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Azure A ≥70% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.

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Naphthol AS-D chloroacetate solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

For use in Sigma Procedure 91C for the demonstration of specific esterase in blood, bone marrow films, tissue touch preparations, cytocentrifuge preparations and paraffin tissue sections.

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Fluorescein Isothiocyanate (mixture of 5- and 6- isomers) ≥90% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

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Alizarin Cyanin Green F ≥60 % (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Green 25, Dye content ≥60 %

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CTK1D7390 97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate is also called as Dil. It can be used to study HCV (Hepatitis C virus) cell interactions in the HCV entry process. It is used to label oxidized LDL (low-density lipoprotein) to quantify the uptake of DiI-oxLDL in human endothelial cells.

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