Estriol 3-sulphate sodium salt ≥98% (by TLC), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Estriol 3-sulfate sodium salt, (1,3,5(10)-Estratriene-3,16alpha,17beta-triol 3-sulfate; 3,16alpha,17beta-Trihydroxy-1,3,5(10)-estratriene 3-sulfate)
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Peroxidase (Myeloperoxidase) Leukocyte Kit Hanker-Yates substrate kit, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Staining system for polymorphonucleocytes in blood or bone marrow films.
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Tissue freezing medium, PolyFreeze, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Tissue samples may be snap frozen using PolyFreeze with isopentane and liquid nitrogen, dry ice (slush/slurry or bunker), or in a cryostat. PolyFreeze media do not cause autofluorescence and can easily be washed away during tissue fixation when rinsed prior to staining. Once rinsed off the slide, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.
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Carbol fuchsin solution, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Intended for use in the Acid Fast Stain, Procedure No. HT80 with Malachite Green counterstain Catalog No. HT80-2
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Eosin Y ∼99% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.
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Haematoxylin (Harris), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
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Gram′s fuchsin solution for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
May contain minor quantities of precipitated dye.
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Chromazurol B
Supplier: SIGMA ALDRICH MICROSCOPY
Chromeazurol B is a dye, belonging to the class of triphenylmethanes.
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Ponceau Xylidine ≥60 % (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Ponceau Xylidine has been used as part of the Goldner’s trichome stain to stain thin sections. It has also been used to prepare Fuchsin-Ponceau solution to stain an amyotrophic lateral sclerosis tissue-engineered
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N,N-Dimethyl-1,4-phenylenediammonium dichloride ≥99.00% (by titrimetric analysis) suitable for peroxidase analysis, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) has been used in the HID-AB (high iron diamine-alcian blue) staining of intestinal tract samples. N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) is used for the determination of peroxidase. DMPD is converted into a red pigment of semiquinone nature. It is also used in the HID-AB (high iron diamine-alcian blue) staining procedures, which are used to differentiate between sialomucin and sulfomucin in the gastrointestinal tract.
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4-Nitrophthalamide 99%, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
4-Nitrophthalamide,99%
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Benzophenone imine ≥95%, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Benzophenone imine plays a vital role as an ammonia surrogate. It is primarily used as ammonia equivalents for the selective formation of protected primary amines. Benzophenone imine is useful as a synthetic intermediate, especially for the synthesis of glycine Schiff base. Benzophenone imine is useful for the preparation of nitrile yield dimers.
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Calcein AM 4 mM in DMSO ≥90% (by HPCE), clear solution
Supplier: SIGMA ALDRICH MICROSCOPY
Calcein AM 4 mM in DMSO ≥90% (by HPCE), clear solution
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Melanin 99% (by TLC), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Melanin is a ubiquitous and prominent pigment responsible for color of skin, hair and eyes. Melanin is classified into eumelanin and pheomelanin. Eumelanin is black to dark brown pigment and is present in black hair and retina of the eye. Pheomelanin is yellow to reddish brown pigment present is red hair and red feathers. Melanins are high molecular weight pigments synthesised from phenolic or indolic precursors and is widely present in animals, plants and microorganisms.
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Eosin Y 0.5 % (w/v) alcoholic solution in acidified ethanol, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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Dextran solution 20% (from Leuconostoc mesenteroides), autoclaved, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Use of dextrans as long and hydrophilic spacer arms to improve the performance of immobilised proteins acting on macromolecules.
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Quinoline yellow sulphonic acid sodium salt (Acid yellow 3) for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Quinoline Yellow,for microscopy (Hist.), mixture of mono- and disulfonic acid sodium salt (Acid Yellow 3)
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Acriflavine fluorescent labeling reagent, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Acriflavine also interacts with HIF-1α (hypoxia-inducible factor 1 α) and HIF-2α, and thereby suppresses dimer formation of HIF-1 and transcriptional function. It also has negative effect on tumor growth and vascularization. Acriflavine also exhibits trypanocidal, antibacterial and antiviral activities.
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Metanil yellow 70% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.
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Bromobimane (mBBr) ≥97%, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.
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Pinacyanol chloride, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Pinacyanol chloride, (1,1'-Diethyl-2,2'-carbocyanine chloride; 2,2'-Trimethinequinocyanine chloride; Quinaldine blue).
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Fluorinert™ FC-770, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluorinert™ belongs to the group of aliphatic compounds. It is considered as a pressure-transmitting medium because it is highly inert. Fluorinert™ Electronic Liquid FC-770 is a thermally stable, fully-fluorinated liquid that has ideal properties for use as a heat transfer fluid in a variety of industries. The inertness of Fluorinert™ FC-770 permits its use as a direct contact single and two-phase coolant in certain supercomputers and sensitive military electronics. Its high dielectric strength and electrical resistivity are ideal for applications in high voltage transformers and power electronics. In the semiconductor manufacturing industry, its wide liquid range makes Fluorinert™ liquid FC-770 suited for cooling ion implanters, dry etchers and CVD machines. Its low pour point also permits its use in thermal shock and test equipment.
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Lucifer yellow CH dilithium salt for fluorescence Stain, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
A highly fluorescent dye, useful in marking nerve cells.
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Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt,powder (Sulfo-SMCC)
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Sulphorhodamine 101 acid chloride, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Sulforhodamine 101 acid chloride has been used in an encapsulated fluorescent probe as a model drug to study drug-delivery properties of rhamnogalacturonan-I based microcapsules. It has also been used in confocal imaging as an astrocyte specific dye.
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Schaeffer and Fulton Spore Stain Solution B, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green (Schaeffer & Fulton`s Spore Stain A: Cat. No. 90903) is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin. The components and the method are also available as kit (Cat. No. 04551).
Schaeffer & Fulton`s Spore Stain B: SafarinO 5 g/l in water
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PAS (Periodic Acid Schiff) staining kit, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Standard procedure and microwave procedure included in package insert.
When treated with periodic acid, glycols are oxidized to aldehydes. After reaction with Schiff′s Reagent (a mixture of pararosaniline and sodium metabisulfite), a pararosaniline adduct
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Crystal violet 1% in aqueous solution, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.
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Zinc protoporphyrin guanylate cyclase inhibitor, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Inhibits soluble guanylate cyclase.