28466 Results for: "G-Biosciences"

Supplier: G-Biosciences

Iodoacetamide

Supplier: G-Biosciences

Ethidium bromide

Supplier: G-Biosciences

GET™ CLEAN DNA uses high binding affinity GET™ spin columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 bp to 20 kb. GET™ spin columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.

Supplier: G-Biosciences

Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

Supplier: G-Biosciences

Swift™ Film Cleaner allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments. Suitable for all exposed film developed for gel shift assays, Western, Southern and Northern blots.

Supplier: G-Biosciences

Triton® X-100 is for solubilising membrane proteins during isolation of membrane-protein complexes.

Supplier: G-Biosciences

Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from E.coli, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

Supplier: G-Biosciences

Dithiothreitol (DTT, Cleland's reagent) 2 mol/l in aqueous solution

Supplier: G-Biosciences

Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

Supplier: G-Biosciences

Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions.

Supplier: G-Biosciences

Chymostatin inhibits serine proteases having a chymotrypsin-like specificity, including α, β γ, and δ chymotrypsin, and most cysteine proteases including cathepsins B, H, L. Soluble in DMSO.

Supplier: G-Biosciences

FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.

Supplier: G-Biosciences

Leupeptin, hemisulphate inhibits serine, plasmin, porcine kallikrein and cysteine proteases, including papain and cathepsin B. Does not inhibit chymotrypsin and thrombin

Supplier: G-Biosciences

Dialysis tubes, Tube-O-DIALYZER™ centrifuge tube adapter

Supplier: G-Biosciences

Genomic Tube‐O‐DIALYZER™ (0.45µm). 

Supplier: G-Biosciences

Big CHAP (N,N-Bis[3-(D-gluconamido)propyl]cholamide)

Supplier: G-Biosciences

Zwitterionic detergent, Aminosulfobetaine with C14 alkyl tail is useful for solubilizing proteins for 2D analysis. Optimal solubility achieved in urea-thiourea mixtures and not in urea alone.

Supplier: G-Biosciences

(3-((3-Cholamidopropyl)dimethylammonio)-2-hydroxy-1-propanesulphonate)

Supplier: G-Biosciences

3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

Immobilised Catalase is prepared by covalent coupling of catalase enzyme to 6% cross-linked agarose beads. Immobilised Catalase is used to remove hydrogen peroxide from biological samples for use in downstream applications which require absenceof hydrogen peroxide as it causes interference.

Supplier: G-Biosciences

Cobalt chelating resin, for the purification of 6X His proteins

Supplier: G-Biosciences

TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether) 10% in water

Supplier: G-Biosciences

Magnetic stand is designed for the efficent pull down of magnetic beads in 1,5 and 2 ml micro centrifgue tubes.

Supplier: G-Biosciences

X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid) is a substrate for ß-glucuronidase (GUS), which is encoded by gusA, a widely used reporter gene. The glucuronidase cleaves X-Gluc to produce colourless glucuronic acid and an intense blue precipitate of chloro-bromoindigo. X-Gluc is used for the histochemical detection of GUS expression in plant cells and tissues, detection of infections caused by E.coli and detection of bacterial contamination in food and water samples.

Supplier: G-Biosciences

OptiBlaze™ ELISA femto-HRP and femto-AP are stabilised ultra sensitive luminol and 1,2 dioxetane based horseradish peroxidase or alkaline phosphatase substrates for the detection of HRP or AP-conjugated antibodies during ELISA.

Supplier: G-Biosciences

These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.

Supplier: G-Biosciences

Column type: Flash/LPLC column, Gravity flow column, total volume 32 ml, resin volume 20 ml, reservoir volume 12 ml, graduated, with screw cap (frit 3 mm ~30 µm PE), Empty columns: Yes, Capacity: 20 ml