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This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.

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An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

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A set of coloured beads that can be designated to represent the Watson-Crick DNA bases: A, T, G, C.

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Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitochondrial DNA and their biological father from chromosomal DNA.

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Single 6-tooth comb, For: Edvotek systems

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Oil spills cause devastation to the environment, killing sea life, birds, and coastal plants. Spraying areas of contamination with oil-eating microbes accelerates the degradation of the oil. This process is known as bioremediation. In this open-ended experiment, students grow a mixture of oil-eating bacteria and observe their effectiveness at degrading a variety of oils.

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According to the World Health Organisation, between two and three million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.

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When normal cells are grown in culture they stop growing when they become overcrowded (contact inhibition). Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumours to form in the body. In addition, many different cell types can be present in a single tumour. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types.

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This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents.

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Electrophoresis Gels, UltraSpec-Agarose™

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Capable of running one or two groups of student samples at the same time and producing excellent results in 30 to 40 minutes.

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FlashBlue™ is a DNA stain that offers simple and rapid staining of agarose gels.

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This lightweight, cordless pipetting controller is ideally suited as an aliquoting tool for instructors and teaching assistants. It uses all standard serological pipettes. The speed can be fine-tuned by applying varying finger pressure to the operating buttons.

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X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

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Educational Materials, Microbiology, Application: Microbiology

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This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.

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The body defends itself from attack by infectious agents like bacteria and viruses by producing antibodies. Enzyme linked immunosorbent assays (ELISA) test for antibodies present in the blood, which indicate infection. In this experiment, students perform a simulated ELISA test to identify infected samples and compare them to control samples.

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Cancer cells differ from normal cells by the combinations of proteins that are present on their surfaces. Antibodies against these proteins will specifically bind to cancer cells and not to normal cells. This allows early detection of cancer and potentially a way of delivering cancer therapies. In this simulation experiment the reaction of cancer cell markers and their corresponding antigens are demonstrated.

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In this experiment, total protein extracts from several bacterial sources are extracted and compared. The unique patterns of protein bands, obtained by SDS vertical polyacrylamide electrophoresis, can be used to identify various bacterial strains.

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Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

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When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.

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The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.

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Water baths incorporating digital temperature control and an optional shaking capacity (10 L model only).

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Universal DNA Buffer TE Buffer PV92 LyphoPrimer Mix LyphoControl EdvoQuick DNA Ladder Proteinase K 1 * 1 KIT

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Bioprocessing is the production and isolation of desired products from living cells. In this introduction to bioprocessing, students will use small-scale fermenters to produce chromogenic proteins using Escherichia coli. Protein extracts will then be 1 * 1 items

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investigation 11: transpiration 1 * 1 items

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what is qpcr and how does it work? 1 * 1 items

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forensics enhancement techniques 1 * 1 items

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ready-to-load dna sequencing 1 * 1 items

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investigation 5: photosynthesis 1 * 1 items