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How Do You Clone a Gene?

How Do You Clone a Gene?

Supplier: EDVOTEK

In this kit, a set of multi-coloured links demonstate a variety of molecular biology simulations. Students learn about digesting DNA with restriction enzymes, cloning genes in plasmids, protein structure, and more.

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Do onions, strawberries and bananas have DNA?

Do onions, strawberries and bananas have DNA?

Supplier: EDVOTEK

Kit allows students to construct DNA models and then extract DNA from onions, strawberries or bananas.

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Kits: the case of the invisible bands, Ready-to-Load™

Kits: the case of the invisible bands, Ready-to-Load™

Supplier: EDVOTEK

Experiment using fluorescence and electrophoresis to solve the mystery of the invisible bands.

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Molecular models: DNA/RNA

Molecular models: DNA/RNA

Supplier: EDVOTEK

Students can build a model of DNA using this simple and colourful system.

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Methylene blue, InstaStain® DNA stain, for electrophoresis

Methylene blue, InstaStain® DNA stain, for electrophoresis

Supplier: EDVOTEK

InstaStain® methylene blue sheets stain gels in minutes and give high quality and uniform staining with excellent results for photography.

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Kits: sickle cell gene detection (DNA-based), Ready-to-Load™

Kits: sickle cell gene detection (DNA-based), Ready-to-Load™

Supplier: EDVOTEK

This Ready-to-Load™ kit shows how a restriction enzyme can be used to screen DNA for Sickle Cell Anaemia.

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Kits: cholesterol diagnostics, Ready-to-Load™

Kits: cholesterol diagnostics, Ready-to-Load™

Supplier: EDVOTEK

Genetic testing can be used to identify people with a genetic condition which caused them to have an elevated level of cholesterol and which can be fatal. Students can see how genetic testing is carried out and learn about DNA electrophoresis.

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Experimentation kits, amplification of DNA by PCR

Experimentation kits, amplification of DNA by PCR

Supplier: EDVOTEK

In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.

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Experimentation kits, PCR-based VNTR human DNA typing

Experimentation kits, PCR-based VNTR human DNA typing

Supplier: EDVOTEK

In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. Using this kit, students type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.

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Nucleic acid testing for COVID-19

Nucleic acid testing for COVID-19

Supplier: EDVOTEK

Hands-on simulation of molecular testing for COVID-19.

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Sequencing the human microbiome

Sequencing the human microbiome

Supplier: EDVOTEK

Learn the basics of DNA sequencing and sequence homology with this lab activity.

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Experimentation kits, simulation of HIV detection by western blot (agarose-based)

Experimentation kits, simulation of HIV detection by western blot (agarose-based)

Supplier: EDVOTEK

The second assay used to confirm a positive HIV ELISA result is the Western blot. Using this kit, students separate protein samples from hypothetical patients on agarose gels. The proteins are then transferred to a membrane and simulated HIV proteins are detected.

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Experimentation kits, survey of protein diversity (polyacrylamide-based)

Experimentation kits, survey of protein diversity (polyacrylamide-based)

Supplier: EDVOTEK

For learning about the diversity of proteins by studying the electrophoretic profiles of various sources. Students separate proteins from bacterial, plant, serum, and milk proteins alongside a standard protein marker.

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Digested lambda DNA

Supplier: EDVOTEK

20 µg for 20 gels.

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Lids for Edvotek digital shaking water baths

Lids for Edvotek digital shaking water baths

Supplier: EDVOTEK

Stainless steel lid, For: 5027

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Experimentation kits, Water Quality Testing III: Multiplex PCR testing of water contaminants

Experimentation kits, Water Quality Testing III: Multiplex PCR testing of water contaminants

Supplier: EDVOTEK

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

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Transformation with green fluorescent protein

Transformation with green fluorescent protein

Supplier: EDVOTEK

In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.

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Kits: analysis of Eco RI cleavage patterns of lambda DNA, Ready-to-Load™

Kits: analysis of Eco RI cleavage patterns of lambda DNA, Ready-to-Load™

Supplier: EDVOTEK

Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.

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Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Supplier: EDVOTEK

When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

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Experimentation kits, in search of the 'Kissing Disease'

Experimentation kits, in search of the 'Kissing Disease'

Supplier: EDVOTEK

Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.

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Experimentation kits, determination of protein molecular weight (polyacrylamide-based)

Experimentation kits, determination of protein molecular weight (polyacrylamide-based)

Supplier: EDVOTEK

Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.

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Ready-to-Load™ Kits, PCR

Ready-to-Load™ Kits, PCR

Supplier: EDVOTEK

Ready-to-Load™ PCR kit introduces students to the principles and applications of the Polymerase Chain Reaction (PCR). This simulation experiment does not contain human DNA and does not require a thermal cycler.

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Experimentation kits, analysis and comparision of mammalian cell types

Experimentation kits, analysis and comparision of mammalian cell types

Supplier: EDVOTEK

Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.

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Single channel pipettes, fixed volume, MiniPipettes

Single channel pipettes, fixed volume, MiniPipettes

Supplier: EDVOTEK

Micropipettes which use standard pipette tips. No need to calibrate and near impossible to measure the wrong volume.

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Microtest tube racks

Supplier: EDVOTEK

Single rack.

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Experimentation kits, water quality testing I: chromogenic analysis of water bacteria contaminants

Experimentation kits, water quality testing I: chromogenic analysis of water bacteria contaminants

Supplier: EDVOTEK

Safe drinking water is vitally important to health. Both pathogenic and harmless bacteria can be found in the guts of mammals and birds. Testing water for every possible type of pathogenic bacteria is slow and costly. Thus, water is tested for a characteristic type of gut bacteria - the coliforms - including the familiar E. coli. Presence of coliforms is an indicator for faecal contamination. In this experiment, students test for coliforms in simulated contaminated water using colour and fluorescent reagents. They can use these same reagents to test water samples from the environment. As an extension activity, a Gram Stain test can be performed on the collected samples.

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Experimentation kits, introduction to plant cell culture

Experimentation kits, introduction to plant cell culture

Supplier: EDVOTEK

Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.

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Experimentation kits, southern blot analysis

Experimentation kits, southern blot analysis

Supplier: EDVOTEK

This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.

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Kits: in search of the cancer gene, Ready-to-Load™

Kits: in search of the cancer gene, Ready-to-Load™

Supplier: EDVOTEK

Suppressor genes, such as p53, are essential for cell functions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations of genes can either be inherited or accumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation.

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Experimentation kits, blood typing

Experimentation kits, blood typing

Supplier: EDVOTEK

ABO and Rh typing of blood left at the scene of a crime can help to narrow down a list of suspects. In this experiment, students use agglutination to identify the blood group of unknown blood samples as a step to identify a criminal.

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