How Do You Clone a Gene?
Supplier: EDVOTEK
In this kit, a set of multi-coloured links demonstate a variety of molecular biology simulations. Students learn about digesting DNA with restriction enzymes, cloning genes in plasmids, protein structure, and more.
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Do onions, strawberries and bananas have DNA?
Supplier: EDVOTEK
Kit allows students to construct DNA models and then extract DNA from onions, strawberries or bananas.
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Kits: the case of the invisible bands, Ready-to-Load™
Supplier: EDVOTEK
Experiment using fluorescence and electrophoresis to solve the mystery of the invisible bands.
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Molecular models: DNA/RNA
Supplier: EDVOTEK
Students can build a model of DNA using this simple and colourful system.
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Methylene blue, InstaStain® DNA stain, for electrophoresis
Supplier: EDVOTEK
InstaStain® methylene blue sheets stain gels in minutes and give high quality and uniform staining with excellent results for photography.
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Kits: sickle cell gene detection (DNA-based), Ready-to-Load™
Supplier: EDVOTEK
This Ready-to-Load™ kit shows how a restriction enzyme can be used to screen DNA for Sickle Cell Anaemia.
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Kits: cholesterol diagnostics, Ready-to-Load™
Supplier: EDVOTEK
Genetic testing can be used to identify people with a genetic condition which caused them to have an elevated level of cholesterol and which can be fatal. Students can see how genetic testing is carried out and learn about DNA electrophoresis.
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Experimentation kits, amplification of DNA by PCR
Supplier: EDVOTEK
In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.
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Experimentation kits, PCR-based VNTR human DNA typing
Supplier: EDVOTEK
In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. Using this kit, students type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.
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Nucleic acid testing for COVID-19
Supplier: EDVOTEK
Hands-on simulation of molecular testing for COVID-19.
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Sequencing the human microbiome
Supplier: EDVOTEK
Learn the basics of DNA sequencing and sequence homology with this lab activity.
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Experimentation kits, simulation of HIV detection by western blot (agarose-based)
Supplier: EDVOTEK
The second assay used to confirm a positive HIV ELISA result is the Western blot. Using this kit, students separate protein samples from hypothetical patients on agarose gels. The proteins are then transferred to a membrane and simulated HIV proteins are detected.
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Experimentation kits, survey of protein diversity (polyacrylamide-based)
Supplier: EDVOTEK
For learning about the diversity of proteins by studying the electrophoretic profiles of various sources. Students separate proteins from bacterial, plant, serum, and milk proteins alongside a standard protein marker.
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Lids for Edvotek digital shaking water baths
Supplier: EDVOTEK
Stainless steel lid, For: 5027
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Experimentation kits, Water Quality Testing III: Multiplex PCR testing of water contaminants
Supplier: EDVOTEK
Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
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Transformation with green fluorescent protein
Supplier: EDVOTEK
In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.
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Kits: analysis of Eco RI cleavage patterns of lambda DNA, Ready-to-Load™
Supplier: EDVOTEK
Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.
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Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase
Supplier: EDVOTEK
When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.
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Experimentation kits, in search of the 'Kissing Disease'
Supplier: EDVOTEK
Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.
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Experimentation kits, determination of protein molecular weight (polyacrylamide-based)
Supplier: EDVOTEK
Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.
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Ready-to-Load™ Kits, PCR
Supplier: EDVOTEK
Ready-to-Load™ PCR kit introduces students to the principles and applications of the Polymerase Chain Reaction (PCR). This simulation experiment does not contain human DNA and does not require a thermal cycler.
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Experimentation kits, analysis and comparision of mammalian cell types
Supplier: EDVOTEK
Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.
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Single channel pipettes, fixed volume, MiniPipettes
Supplier: EDVOTEK
Micropipettes which use standard pipette tips. No need to calibrate and near impossible to measure the wrong volume.
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Experimentation kits, water quality testing I: chromogenic analysis of water bacteria contaminants
Supplier: EDVOTEK
Safe drinking water is vitally important to health. Both pathogenic and harmless bacteria can be found in the guts of mammals and birds. Testing water for every possible type of pathogenic bacteria is slow and costly. Thus, water is tested for a characteristic type of gut bacteria - the coliforms - including the familiar E. coli. Presence of coliforms is an indicator for faecal contamination. In this experiment, students test for coliforms in simulated contaminated water using colour and fluorescent reagents. They can use these same reagents to test water samples from the environment. As an extension activity, a Gram Stain test can be performed on the collected samples.
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Experimentation kits, introduction to plant cell culture
Supplier: EDVOTEK
Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.
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Experimentation kits, southern blot analysis
Supplier: EDVOTEK
This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.
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Kits: in search of the cancer gene, Ready-to-Load™
Supplier: EDVOTEK
Suppressor genes, such as p53, are essential for cell functions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations of genes can either be inherited or accumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation.
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Experimentation kits, blood typing
Supplier: EDVOTEK
ABO and Rh typing of blood left at the scene of a crime can help to narrow down a list of suspects. In this experiment, students use agglutination to identify the blood group of unknown blood samples as a step to identify a criminal.