Mouse TNF alpha ELISA kit is a high sensitivity sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse TNF alpha in serum, plasma, and other biological fluids.

• High Sensitivity: Detection at the picogram level!
• Assay Precision: Intra-Assay: CV <10%, Inter-Assay: CV <10%

Mouse TNF alpha ELISA kit employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse TNF alpha in serum, plasma, and other biological fluids. An antibody specific for TNF alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TNF alpha present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-TNF alpha Antibody, which binds the captured TNF alpha present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TNF alpha captured in each well. The concentration of TNF alpha can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.