AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

• Optimized buffer chemistry destabilizes single base-pair mismatch probes, providing superior allelic discrimination and improved cluster separation for SNP detection assays
• ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors
• Easy-to-use 2X concentrated SuperMix with AccuVue plate loading dye

AccuStart™ Genotyping ToughMix® contains all required reaction components, except primers, probes, and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product. AccuStart™ Genotyping ToughMix® is compatible with both fast and conventional PCR cycling protocols.