- Description:EasySep™ human central and effector memory CD8+ T cell isolation kit, 1×10⁹ cells
- Environmentally Preferable:
- Pk:1 KIT
Immunomagnetic positive selection of human central and effector memory CD8+ T cells.
- Easy-to-use and column-free
- Up to 93% purity
- No-wash removal of EasySep™ Releasable RapidSpheres™
This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD8+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD8+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD8+ T cells that are immediately ready for downstream applications. Effector memory CD8+ T cells can be isolated from the CD62L- fraction.