Column-free immunomagnetic positive selection of human CD3+ cells for HLA analysis.
- Fast and easy-to-use
- Up to 99% purity
- No columns required
- Compatible across EasySep™ "The Big Easy", EasyEights™, and RoboSep™-S platforms
The EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit is designed to isolate CD3+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognising CD3 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimise nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T cells and NKT cells.
This product replaces the EasySep™ Human Whole Blood CD3 Positive Selection Kit (STMC18081) and EasySep™ HLA Whole Blood CD3 Positive Selection Kit (STMC18081HLA), for even faster cell isolations.