Mouse HSP70 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse HSP70 in serum, plasma, tissue homogenates, and other biological fluids.

• Ready-to-use ELISA kit
• Detection range: 39,063 to 2500 pg/ml
• Sensitivity: 23,438 pg/ml
• Sample type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra to Assay: CV <8%, Inter to Assay: CV <10%

Mouse HSP70 ELISA Kit (A74758) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse HSP70 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for HSP70 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HSP70 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HSP70 Antibody, which binds the captured HSP70 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HSP70 captured in each well. The concentration of HSP70 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.