Mouse TSH beta ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse TSH beta in serum, plasma, tissue homogenates, and other biological fluids.

• Ready To Use ELISA kit
• Detection Range: 0,156 to 10 µIU/ml
• Sensitivity: 0,094 µIU/ml
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Mouse TSH beta ELISA kit (A78929) employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse TSH beta in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with TSH beta antigen. During the incubation, TSH beta present in the samples or standards competes with the fixed amount of immobilized TSH beta for binding sites on the Biotinylated Anti-TSH beta Antibody. The more TSH beta present in a sample or standard, the less Biotinylated Anti-TSH beta Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-TSH beta Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of TSH beta present in each sample or standard. The concentration of TSH beta can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.