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Mouse B4GALT7 ELISA Kit
Mouse B4GALT7 ELISA Kit
  ANTIA303499-96
 :  Antibodies.com
New Product
 :  
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Mouse B4GALT7 ELISA Kit
  ANTIA303499-96
 :  Antibodies.com
New Product
 :  

 

  • Pk:
    96 Tests
  • Assay duration:
    Multiple steps
  • Assay Type:
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Mouse
  • Target protein:
    B4GALT7
  • Description:
    Mouse B4GALT7 ELISA kit
  • Sample type:
    Serum, plasma, tissue homogenates, and other biological fluids
  • Cross reactivity:
    Mouse B4GALT7 ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse B4GALT7. No significant cross-reactivity or interference between B4GALT7 and analogues was observed.
  • Detection method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf life:
    Store for 6 months at 4 °C
  • Detection range:
    78,125 - 5000 pg/ml
  • Storage temperature:
    4 °C
  • Sensitivity:
    46,875 pg/ml
  • Regulatory status:
    RUO

 

 

Mouse B4GALT7 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse B4GALT7 in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready to use ELISA kit
  • Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Mouse B4GALT7 ELISA kit (A303499) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse B4GALT7 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for B4GALT7 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the B4GALT7 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-B4GALT7 antibody, which binds the captured B4GALT7 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of B4GALT7 captured in each well. The concentration of B4GALT7 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.