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Mouse IgG2a ELISA Kit
Mouse IgG2a ELISA Kit
  ANTIA75515-96
 :  ANTIBODIES.COM
New Product
 :  
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Mouse IgG2a ELISA Kit
  ANTIA75515-96
 :  ANTIBODIES.COM
New Product
 :  

 

  • Pk:
    96 Tests
  • Assay duration:
    Multiple steps
  • Assay Type:
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Mouse
  • Target protein:
    IgG2a
  • Description:
    Mouse IgG2a ELISA kit
  • Sample type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross reactivity:
    Mouse IgG2a ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse IgG2a. No significant cross-reactivity or interference between IgG2a and analogues was observed.
  • Detection method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf life:
    Store for 6 months at 4 °C
  • Detection range:
    0,781 - 50 ng/ml
  • Storage temperature:
    4 °C
  • Sample volume:
    100 μl
  • Sensitivity:
    0,469 ng/ml
  • Regulatory status:
    RUO

 

 

Mouse IgG2a ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IgG2a in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready to use ELISA kit
  • Detection range: 0,781 to 50 ng/ml
  • Sensitivity: 0,469 ng/ml
  • Sample type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Mouse IgG2a ELISA Kit (A75515) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse IgG2a in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for IgG2a has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IgG2a present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IgG2a Antibody, which binds the captured IgG2a present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IgG2a captured in each well. The concentration of IgG2a can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.