- Antibody type:Primary
- Antigen name:immunoglobulin heavy constant mu
- Antigen symbol:IGHM
- Clonality:Monoclonal
- Clone:MuHC2
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1 kappa
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P01871
- Antigen synonyms:IGHM
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Human Mu heavy chain was used as the immunogen for this IgM antibody.
- Purification:Protein G
- Size:100 µg
- Pk:100 µG
IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body.
Immunofluorescence: 0,5 - 1 µg/ml Western blot: 0,5 - 1 µg/ml IHC (FFPE): 0,5 - 1 µg/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.
1. FFPE staining requires boiling sections in 10 mM citrate buffer, pH 6,0, for 10 - 20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: IGHM
Clonality: Monoclonal
Clone: MuHC2
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human