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Human recombinant pyruvate kinase M2 (from E. coli)
Catalog # PRSI92-296
Supplier:  ProSci Inc.
New Product
CAS Number:  
Human recombinant pyruvate kinase M2 (from E. coli)
Catalog # PRSI92-296
Supplier:  ProSci Inc.
New Product
CAS Number:  

Specifications

  • Pk:
    0,05 mg
  • Enzyme type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Environmentally Preferable:
  • Tag sequence:
    N-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt, please minimise freeze - thaw cycles
  • Endotoxin content:
    <0,1 ng/µg (1 IEU/µg) as determined by LAL test
  • Enzyme name:
    Pyruvate Kinase M2
  • Enzyme synonyms:
    Pyruvate kinase PKM, CTHBP, OIP-3, THBP1, PKM2
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    60,1 kD
  • Sequence:
    Ser2-Pro531
  • Formulation:
    Supplied as a 0,2 µm filtered solution of PBS, pH 7,0, 10% glycerol. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilised protein in ddH₂O. Please aliquot the reconstituted solution to minimise freeze-thaw cycles.
  • Tested applications:
    Biological assays

Specifications

About this item

Pyruvate kinase isoform M2 (PKM2) is a member of the pyruvate kinase (PK) family, a pivotal glycolytic enzyme that is consistently changed during tumorigenesis.

This recombinant protein can be used for biological assays. For research use only.

Fusion-Tag: N-6 His tag

PKM2 is expressed in some differentiated tissues, such as lung, fat tissue, retina, and pancreatic islets. As the rate-controlling enzyme in glycolysis, PKM2, has also been found to be expressed in embryonic, proliferating, and tumor cells, and it has been considered to be crucial for the metabolism and growth of tumor cells. Recent studies have shown that PKM2 promotes cell proliferation and suppresses cell apoptosis in various tumors. In addition, it has been reported that the PKM2 knockdown affects the Akt and ERK protein kinases.