Specifications

• Pk:
  100 µG
• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Mouse
• Environmentally Preferable:
• Tag sequence:
  His Tag
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Measured by its binding ability in a functional ELISA. Immobilised Mouse Nectin-4, His Tag (Cat. No. NE4-M52H3) at 5 μg/ml (100 μl/well) can bind Human PVRL1 / NECTIN1 Protein, Fc Tag with a linear range of 0,039 - 1,25 μg/ml (QC tested). Loaded Mouse Nectin-4, His Tag (Cat. No. NE4-M52H3) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 0,12 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  Q8R007-1
• Protein synonyms:
  NECTIN4|PRR4|PVRL4|LNIR
• Protein/peptide name:
  Nectin-4
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular weight:
  36,2 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, Arginine, pH 7,4. Normally trehalose is added as protectant before Lyophilisation.
• Shipping temperature:
  RT