Specifications

• Pk:
  50 µG
• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Mouse
• Environmentally Preferable:
• Tag sequence:
  His Tag (SPR & BLI verified)
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Immobilised Mouse CD32b, His Tag (SPR & BLI verified) (ACRBCDB-M52H7) on NTA-Series S sensor chip, can bind OKT3 with an affinity constant of 0.86 μM as determined in a SPR assay (Biacore T200) (QC tested). Immobilised OKT3 on Protein G-Series S sensor chip, can bind Mouse CD32b, His Tag (SPR & BLI verified) (ACRBCDB-M52H7) with an affinity constant of 0,55 μM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Mouse CD32b, His Tag (SPR & BLI verified) (ACRBCDB-M52H7) on NTA Biosensor, can bind OKT3 with an affinity constant of 1,2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Mouse CD32b, His Tag (SPR & BLI verified) (ACRBCDB-M52H7) on NTA Biosensor, can bind Herceptin with an affinity constant of 2,1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded OKT3 on Protein G Biosensor, can bind Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) with an affinity constant of 1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Herceptin on Protein G Biosensor, can bind Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) with an affinity constant of 1,2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  P08101
• Protein synonyms:
  CD32|IGFR2|CD32b|FCG2|CDw32|FCGR2B
• Protein/peptide name:
  Fc gamma RIIB/ CD32b
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular weight:
  22,2 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH7,4. Normally trehalose is added as protectant before lyophilisation.
• Shipping temperature:
  RT