Specifications

• Pk:
  25 µG
• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Environmentally Preferable:
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.; Measured by its binding ability in a functional ELISA. Immobilized Human IL-12B and IL-12A heterodimer protein, His Tag and Flag Tag (Cat. No. ACRBIL2-H4210) at 0,5 μg/ml (100 μl/well) can bind anti-IL23A and IL12B MAb (P40 domain) with a linear range of 0,2 - 2 ng/ml (QC tested). Measured by its binding ability in a functional ELISA. Immobilised biotinylated human IL-12 R beta 1, Fc,Avitag (Cat. No. ACRBILB-H82F7) at 1 μg/ml (100 μl/well) on streptavidin (Cat. No. ACRBSTN-N5116) precoated (0,5 μg/well) plate, can bind human IL-12B and IL-12A heterodimer protein, His Tag and Flag Tag (Cat. No. ACRBIL2-H4210) with a linear range of 0,039 - 0,625 μg/ml (routinely tested). Human IL-12 R beta 1, Fc Tag (Cat. No. ACRBILB-H5255) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B and IL-12A heterodimer protein, His tag and flag tag (Cat. No. ACRBIL2-H4210) with an affinity constant of 2,06 nM as determined in a SPR assay (Biacore T200) (routinely tested).; Loaded Anti-IL23A and IL12B MAb (P40 domain) on protein A biosensor, can bind Human IL-12B and IL-12A heterodimer protein, His tag and flag tag (Cat. No. ACRBIL2-H4210) with an affinity constant of 1,07 nM as determined in BLI assay (ForteBio Octet Red96e) (routinely tested). Loaded human IL-12B and IL-12A heterodimer protein, His Tag and Flag Tag (Cat. No. ACRBIL2-H4210) on HIS1K Biosensor, can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with an affinity constant of 92.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Human IL-12B and IL-12A Heterodimer Protein, His Tag and Flag Tag (Cat. No. ACRBIL2-H4210) stimulates the proliferation of PHA-P activated PBMC. The ED50 for this effect is 0,05 ng/ml (routinely tested).
• Gene ID:
  P29460-1 (IL12B) and P29459-1 (IL12A)
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  IL12|p70|Interleukin-12
• Protein/peptide name:
  IL-12B and IL-12A
• Purity:
  >90%
• Molecular weight:
  36,6 kDa (IL12B) and 23,8 kDa (IL12A)
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT