Visinin (sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1) was originally isolated biochemically from chicken retina as a major protein of about 24kDa on SDS-PAGE. Following cloning and sequencing of visinin, several visinin like proteins were discovered by homology screening. One of these, Visinin-like protein 1 is a small Calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein belongs to the large superfamly of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the confomation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four 'EF hand' domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding. Visinin-like protein 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of 22kDa.

• The antibody reacts with a 22 kDa band by Western blot on bovine cerebellum lysate

Application information: Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:500 to 1:1,000 is recommended for WB. A dilution of 1:500 to 1:1,000 is recommended for IHC and ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: VISL1
Clonality: Monoclonal
Clone: 2D11
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human, Rat, Mouse, Cow