A sensitive detection method for measuring the free acid form of fluprostenol.

• Measure the free acid form of fluprostenol
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure fluprostenol levels down to 16 pg/ml
• Colorimetric reading at 405 to 420 nm

Fluprostenol is a metabolically stable analog of PGF_2α with potent FP receptor agonist activity. The isopropyl ester of fluprostenol has recently been approved for use as an ocular hypotensive drug for the treatment of glaucoma and is sold under the Alcon trade name Travoprost. Acting as a prodrug, Fluprostenol isopropyl ester is converted by esterase activity in the cornea to yield the corresponding active free acid, (+)-fluprostenol. Fluprostenol is also used in the veterinary field for the induction of estrus in livestock. Cayman’s Fluprostenol ELISA is a sensitive detection method for measuring the free acid form of fluprostenol. The assay also detects the isopropyl ester form of fluprostenol, but with less sensitivity. Samples containing a mixture of the isopropyl ester and free acid should be purified and the compounds separated prior to analysis. The assay has been validated for use in aqueous humor samples.