Human recombinant BDNF (from E. coli)

Supplier: Shenandoah Biotechnology

Certificates

Supplier: Shenandoah Biotechnology

About this item

Brain-derived neurotrophic factor (BDNF) is a nerve growth factor that binds two receptors, the low-affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase B (TrkB), to support neuron growth and survival.

Produced from genetically modified E. coli
Noncovalent homodimeric protein, containing 120/240 amino acids

BDNF expression in the hippocampus is essential for long-term memory storage and learning. Human, mouse, rat, and pig BDNF are cross-reactive.

Delivery: A certificate of analysis (COA) is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions.

Caution: This product is for research use only and is not for use in humans.

Specifications

Conjugation:
Unconjugated
Protein function:
Cytokine
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
–20 °C
Endotoxin content:
Endotoxin LAL (EU/µg) ≤0,1
Biological activity:
Bioactivity assay: Proliferation of a neuroblastoma cell line stably expressing TrkB (BR6), ED50 ≤40 ng/ml, Bioactivity ≥2,5×10⁴ units/mg
Gene ID:
P23560
Reconstitution instructions:
Sterile water at 0.1 mg/ml
Endotoxin-free:
N
Carrier-free:
Y
Protease-free:
N
Animal-free:
N
Protein synonyms:
ANON2|brain-derived neurotrophic factor|BNDF|BULN2
Protein/peptide name:
BDNF
Purity:
≥95%
Molecular weight:
13,6/27,3 kDa
Sequence:
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Endotoxin level:
Low
Formulation:
0,1% Trifluoroacetic Acid (TFA)
Nuclease-free:
N
Grade:
RUO
Shipping temperature:
RT
Tested applications:
Bioassay