Nuclear extract from HeLa cells
ENZOBMLKI1400100
:
- Pk:100 µl
- Description:Nuclear extractor
- Seq:02
- Storage buffer:Liquid. In 0,1 M KCl, 20 mM HEPES/NaOH, pH 7,9, 20% (v/v) glycerol, 0,2 mM EDTA, 0,5 mM DTT, 0,5 mM PMSF.
Prepared by high salt extraction of HeLa nuclei (human cervical cancer cell line), this extract is a rich source of HDAC activity, useful for inhibitor screening or immunoprecipitation of HDAC containing complexes. Typically, 0,5 µl or less per well is sufficient for assay with the HDAC FLUOR DE LYS® fluorometric activity assay.