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Human recombinant Carbonic Anhydrase 1 (from E. coli)
Human recombinant Carbonic Anhydrase 1 (from E. coli)
  PRSI91-090
 :  
Human recombinant Carbonic Anhydrase 1 (from E. coli)
  PRSI91-090
 :  

 

  • Pk:
    50 µG
  • Enzyme type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Environmentally Preferable:
  • Tag sequence:
    C-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Enzyme name:
    Carbonic Anhydrase 1
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    29.93 kD
  • Sequence:
    Ala2-Phe261
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10% Glycerol, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    For Biological assays

 

 

Carbonic Anhydrase 1 (CA1) is a cytosolic enzyme, belonging to the alpha-carbonic anhydrase family. It is highly expressed in erythrocytes and acts as an early marker for erythroid differentiation. Carbonic anhydrase 1 plays a improtant role in many biological processes such as calcification, cellular respiration, bone resorption, acid-base balance. It is activated by imidazole, histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. At the same time, It is inhibited by sulfonamide derivatives and coumarins. In addition, CA1 is a zinc metalloenzyme that has reversible hydration of carbon dioxide. It can hydrate cyanamide to urea.

Fusion-Tag: C-6 His tag

This recombinant protein can be used for biological assays. For research use only.