Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules. Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions.

• Purifies biotinylated products using mild elution conditions
• Monomeric avidin resin can be regenerated and reused at least 10 times
• Retains biotin-binding specificity and low non specific binding of native avidin
• Bind samples in a variety of physiological buffers
• Binding capacity > than 1,2 mg biotinylated BSA/ml resin

During monomeric avidin immobilisation, polymeric forms of avidin with strong binding characteristics also are immobilized. These high affinity biotin-binding sites must first be blocked with a biotin-containing buffer. Biotin molecules are eluted from monomers with a glycine solution revealing only the reversible binding sites. The biotinylated molecule of interest may then be applied to the support for purification and then eluted by ligand competition using a biotin-containing solution.