Pierce™ streptavidin coated PS microplates are ideal for binding assays using biotinylated antibodies. High binding capacity (HBC) plates provide a wider detection range and better curve linearity for small biotinylated ligands, such as peptides and oligonucleotides, than the standard coated plates. Available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays.

• Low non specific binding
• Easy and gentle immobilisation of biotin-containing conjugates
• No denaturing of the protein component of a conjugate upon binding
• Pre-blocked with Blocker™ BSA or SuperBlock™ blocking buffer and ready-to-use
• High binding capacity plates provides wider detection range and better curve linearity for small biotinylated ligands

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.