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NGF Rapid™ ELISA kit: rat
NGF Rapid™ ELISA kit: rat
Supplier:  Biosensis
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NGF Rapid™ ELISA kit: rat
Supplier:  Biosensis

Specifications

  • Assay Type:
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Rat
  • Target protein:
    NGF
  • Cross reactivity:
    The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5 and proNGF protein tested at 25 ng/ml in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse. Mature NGF (27 kDa) and full-length proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the NGF ELISA calibration range (3,9-250 pg/ml; 0,14-9,2 pmol/l). OD readings for proNGF were indistinguishable from the assay’s blank OD readings. Data was obtained with the Mouse NGF ELISA kit which uses the same assay capture and detection antibodies.
  • Detection range:
    3,9 – 250 pg/ml
  • Sensitivity:
    Typical limit of detection (LOD) for rat NGF is 8 pg/ml determined as 150% of the blank value

Specifications

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