The Biosensis proNGF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and lysates only if used as directed.

The Rapid™ proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognise proNGF, the pro-domain peptide, but do not cross-react with mature NGF.

The purpose of this kit is the in vitro quantitative determination of proNGF in cell culture supernatants and human serum. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike and recovery experiments in order to validate this ELISA assay for other sample matrices, if required.

Note that accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker provided in the kit, and available for purchase separately.

This kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A human proNGF positive control (QC sample) is provided to assure consistent assay performance.

Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples.