Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.

For affinity chromatography
Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
Convenient purification of histidine-tagged proteins, also when Ni2+ is not the best choice of metal ion
Stable in commonly used aqueous buffers 0.01 M HCl, 1 M NaOH, 70% acetic acid, 2% SDS. 30% 2-propanol

IMAC Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification. As a BioProcess medium, IMAC Sepharose 6 Fast Flow fulfills industrial demands for security of supply, robust performance and regulatory support. The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.

IMAC Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, IMAC Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

IMAC Sepharose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein

: Available in the convenient and time-saving prepacked HiTrap and HiPrep formats.

pH range:
3 - 12
Matrix:
6% cross-linked agarose
Storage temperature:
4 - 30 °C
Binding capacity:
∼40 mg histidine-tagged protein/ml medium when changed with Ni2⁺2)
Total ionic capacity:
~25 µmol Cu2₊/ml
~15 µmol Ni2₊ or Zn2₊/ml