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Electrophoresis Reagents

Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Amersham ECL Direct Labeling System, Cytiva

Amersham ECL Direct Labeling System, Cytiva

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

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Substrate for Western blotting, ECF™

Supplier: Cytiva

ECF™ substrate for Western blotting

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Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia

Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia

Supplier: Cytiva

Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

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Cy™ fluorescent amidites

Cy™ fluorescent amidites

Supplier: Cytiva

Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.

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Western blotting detection reagent, Amersham™ ECL™ start

Western blotting detection reagent, Amersham™ ECL™ start

Supplier: Cytiva

Chemiluminescence detection reagent for detection of high to medium abundant proteins.

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CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva

Supplier: Cytiva

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.

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Western blotting system, Amersham ECL™

Western blotting system, Amersham ECL™

Supplier: Cytiva

Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.

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Antibody diluent, MP Biomedicals

Supplier: MP Biomedicals

This antibody diluent is compatible with common stabilising compounds at pH 7,3.

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Amersham™ ECL Direct™ Detection Reagents, Cytiva

Amersham™ ECL Direct™ Detection Reagents, Cytiva

Supplier: Cytiva

Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.

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Western blotting blocking reagent, Amersham ECL™ Prime

Western blotting blocking reagent, Amersham ECL™ Prime

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

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Amersham ECL Direct Labeling and Detection System, Cytiva

Amersham ECL Direct Labeling and Detection System, Cytiva

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

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pI markers

pI markers

Supplier: Cytiva

pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.

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Protein and peptide molecular weight markers

Protein and peptide molecular weight markers

Supplier: Cytiva

For determination of molecular weight of uncharacterised protein samples with precision by comparison with carefully selected standards.

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Molecular weight markers, Rainbow™

Molecular weight markers, Rainbow™

Supplier: Cytiva

Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.

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Protein standards

Protein standards

Supplier: Genscript

GenScript offers a wide selection of Protein molecular weight standards for SDS-PAGE and Western blot, including prestained, unstained, peptide, Western-viewable, and other standards.

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Western blotting markers, Amersham™ ECL™ DualVue™

Western blotting markers, Amersham™ ECL™ DualVue™

Supplier: Cytiva

Amersham™ ECL™ DualVue™ Western blotting markers perform two key functions. Firstly, the prestained marker proteins confirm protein transfer in addition to clearly defining blot orientation. Secondly, the recombinant tagged proteins are detected on film or by CCD imaging in conjunction with the target protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target protein(s) and markers.

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Molecular weight markers, Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers

Molecular weight markers, Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers

Supplier: Cytiva

Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers are protein molecular weight markers optimised for use with the ECL™ Plex™ Western blotting detection system. They are designed to provide visible marker bands on gels and membranes, as well as fluorescent images using Cy3 and Cy5 channels.

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BCIP®/NBT alkaline phosphatase substrate, SIGMAFAST™

Supplier: Merck

SIGMAFAST™ BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) tablets have been developed for use in immunochemistry as a precipitating substrate for the detection of alkaline phosphatase activity.

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Liquid scintillation cocktail, Ultima Gold™

Supplier: PerkinElmer

Liquid scintillation cocktail, Ultima Gold™, 2×5 l

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IEF carrier ampholytes, SERVALYT™

Supplier: Serva

IEF carrier ampholyte, SERVALYT™, pH 6 - 7, analytical grade

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DNA Ladder 1 kb

DNA Ladder 1 kb

Supplier: STEMCELL Technologies

DNA ladder consisting of 13 fragments from 250 to 10000 base pairs for determining the size of double-stranded DNA by gel electrophoresis.

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