Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Amersham ECL Direct Labeling System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
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Substrate for Western blotting, ECF™
Supplier: Cytiva
ECF™ substrate for Western blotting
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Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia
Supplier: Cytiva
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
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Cy™ fluorescent amidites
Supplier: Cytiva
Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.
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Western blotting detection reagent, Amersham™ ECL™ start
Supplier: Cytiva
Chemiluminescence detection reagent for detection of high to medium abundant proteins.
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CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva
Supplier: Cytiva
CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.
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Western blotting system, Amersham ECL™
Supplier: Cytiva
Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.
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Antibody diluent, MP Biomedicals
Supplier: MP Biomedicals
This antibody diluent is compatible with common stabilising compounds at pH 7,3.
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Amersham™ ECL Direct™ Detection Reagents, Cytiva
Supplier: Cytiva
Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.
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Western blotting blocking reagent, Amersham ECL™ Prime
Supplier: Cytiva
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
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Amersham ECL Direct Labeling and Detection System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
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pI markers
Supplier: Cytiva
pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.
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Protein and peptide molecular weight markers
Supplier: Cytiva
For determination of molecular weight of uncharacterised protein samples with precision by comparison with carefully selected standards.
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Molecular weight markers, Rainbow™
Supplier: Cytiva
Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.
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Protein standards
Supplier: Genscript
GenScript offers a wide selection of Protein molecular weight standards for SDS-PAGE and Western blot, including prestained, unstained, peptide, Western-viewable, and other standards.
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Western blotting markers, Amersham™ ECL™ DualVue™
Supplier: Cytiva
Amersham™ ECL™ DualVue™ Western blotting markers perform two key functions. Firstly, the prestained marker proteins confirm protein transfer in addition to clearly defining blot orientation. Secondly, the recombinant tagged proteins are detected on film or by CCD imaging in conjunction with the target protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target protein(s) and markers.
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Molecular weight markers, Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers
Supplier: Cytiva
Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers are protein molecular weight markers optimised for use with the ECL™ Plex™ Western blotting detection system. They are designed to provide visible marker bands on gels and membranes, as well as fluorescent images using Cy3 and Cy5 channels.
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BCIP®/NBT alkaline phosphatase substrate, SIGMAFAST™
Supplier: Merck
SIGMAFAST™ BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) tablets have been developed for use in immunochemistry as a precipitating substrate for the detection of alkaline phosphatase activity.
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Liquid scintillation cocktail, Ultima Gold™
Supplier: PerkinElmer
Liquid scintillation cocktail, Ultima Gold™, 2×5 l
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IEF carrier ampholytes, SERVALYT™
Supplier: Serva
IEF carrier ampholyte, SERVALYT™, pH 6 - 7, analytical grade
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DNA Ladder 1 kb
Supplier: STEMCELL Technologies
DNA ladder consisting of 13 fragments from 250 to 10000 base pairs for determining the size of double-stranded DNA by gel electrophoresis.