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You searched for: Electrophoresis Buffers

Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

VWR® Transfer Buffer Concentrate, CAPS 10X

VWR® Transfer Buffer Concentrate, CAPS 10X

Supplier: VWR Chemicals

For transfer of protein to membranes for sequencing or blotting.

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VWR® Gentle ReView™, Stripping Buffer

VWR® Gentle ReView™, Stripping Buffer

Supplier: VWR Chemicals

Gentle ReView™ Stripping Buffer is a mild, yet effective method for stripping primary and secondary antibodies from PVDF or nitrocellulose membranes.

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VWR®, MOPS 10X buffer for biotechnology

VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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Phosphate Buffer, pH 6.6 at 25 °C

Supplier: SIGMA ALDRICH MICROSCOPY

Phosphate buffer's buffering capacity is pH sensitive and is preferred to be used at physiological pH (7,2 to 7,4).

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VWR®, Tris-borate-EDTA (TBE) buffer

VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

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VWR® Proteomics Grade, Tris-glycine buffer

VWR® Proteomics Grade, Tris-glycine buffer

Supplier: VWR Chemicals

A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

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VWR®, SSC buffer, 20X, ultra-pure grade

VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR®, Rapid Transfer buffer 10X

VWR®, Rapid Transfer buffer 10X

Supplier: VWR Chemicals

Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

    
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VWR® Phosphate Buffered Saline (PBS), Low Phosphate

VWR® Phosphate Buffered Saline (PBS), Low Phosphate

Supplier: VWR Chemicals

Irradiated solution for use in washing cells.

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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

Supplier: VWR Chemicals

Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

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VWR® UltraPure, PBS and TBS wash buffers

VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR® Phosphate Buffered Saline (PBS), High Phosphate

VWR® Phosphate Buffered Saline (PBS), High Phosphate

Supplier: VWR Chemicals

Irradiated solution, pH 7,2, for washing blots.

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VWR® Buffer, MES-SDS, 20X, UltraPure Grade

VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X

VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X

Supplier: VWR Chemicals

Irradiated concentrate of PBS, suitable for use in washing cells after dilution.

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VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20

VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.

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VWR® Separating Gel Buffer, 4X

VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

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VWR®, Blocking buffer, non-fat powdered milk

VWR®, Blocking buffer, non-fat powdered milk

Supplier: VWR Chemicals

Each pack of powdered blocking buffer makes one liter of 1X solution.

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VWR®, Hybridisation buffers with 50% formamide

VWR®, Hybridisation buffers with 50% formamide

Supplier: VWR Chemicals

Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.

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VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade

VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade

Supplier: VWR Chemicals

PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.

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2X SDS-PAGE sample buffer without DTT or b-ME

Supplier: Rockland Immunochemicals

Sample buffer for protein electrophoresis.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: G-Biosciences

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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IHC Wash Buffer, Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

The IHC wash buffer is a for washing and removing excess reagents during immunostaining.

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VWR®RapidBlock™, Blocking solution, 10x

VWR®RapidBlock™, Blocking solution, 10x

Supplier: VWR Chemicals

RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.

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Electrophoresis running buffers

Electrophoresis running buffers

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

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Phosphate buffered saline (PBS), 10X

Phosphate buffered saline (PBS), 10X

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Rockland Immunochemicals

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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